A strain of Bacillus thuringiensis degrading pesticides and its application
A technique for Bacillus aureus and pesticide degradation, applied in the field of environmental microorganisms and agriculture, to achieve strong environmental adaptability, protection of the ecological environment and human health, and high safety effects
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Embodiment 1
[0024] The acquisition of embodiment 1 Bacillus thuringiensis Bt-1
[0025] The present inventor screened and obtained Bacillus thuringiensis Bt-1 by enrichment culture method. Soil samples used for enrichment were collected from Singapore. Get soil sample 5g and add 50mL basal medium (formulation is: (NH 4 ) 2 8O 4 , 2g; MgSO4, 0.2g; CaCl 2 , 0.01g; FeSO 4 , 0.005g; MnCl 2 , 0.002g; K 2 HPO 4 , 10.5g; KH 2 PO 4 , 4.5g, in distilled water 1000mL, pH7.5). At the same time, the mother solution of azoxystrobin original drug (DMSO as solvent) was added to make the final mass concentration of azoxystrobin 50 mg / L, and the degrading bacteria enrichment culture was carried out at 30°C and 150 r / min on a shaking table. After culturing for 7 days, they were transferred to the second batch of basal medium containing 100 mg / L azoxystrobin at an inoculum size of 10%. After culturing under the same conditions for 7 days, the 10% inoculum was transferred to the basal medium cont...
Embodiment 2
[0026] The identification of embodiment 2 Bacillus thuringiensis Bt-1
[0027] The bacterial strain is light yellow in the LB solid plate, and the colonies are large, with irregular edges and spores. Through the determination of physiological and biochemical characteristics, it is shown that the strain belongs to Gram-positive bacteria; it can utilize glucose, glycerol, ribose, fructose, maltose, and starch; it does not utilize xylose, galactose, mannitol, rhamnose, lactose, trehalose, Arabic candy. The GenBank accession number of the strain 16SrDNA is KF290569. Therefore, the strain Bt-1 was identified as Bacillus thuringiensis by morphological characteristics, physiological and biochemical characteristics and 16S rDNA phylogenetic analysis.
[0028] The strain was deposited in the China Center for Type Culture Collection (CCTCC), the preservation date was December 20, 2013, and the preservation number was CCTCC M 2013680, and the strain was named Bacillus thuringiensis str...
Embodiment 3
[0030] Embodiment 3 Bacillus thuringiensis Bt-1 is to the biodegradation effect of different pesticides
[0031] Add azoxystrobin, kresstrobin, pyraclostrobin, beta-cypermethrin, deltamethrin, beta-cyhalothrin and fenpropathrin respectively in the basal medium (the formula is the same as in Example 1), so that The final concentration is 50mg / L; the inoculum concentration is 1.0×10 7 CFU / mL of the bacterial agent, and set the medium without inoculation as the control, culture at 31°C for 0-5 days, take samples regularly, use HPLC to detect the pesticide residues and calculate the degradation rate, the results are shown in Table 1. The results showed that Bacillus thuringiensis Bt-1 could effectively degrade azoxystrobin, kresoxystrobin, pyraclostrobin, lambda-cypermethrin, deltamethrin, lambda-cyhalothrin and fenpropathrin in a short time, The degradation rates of the above pesticides reached 82.5%, 86.2%, 85.4%, 97.1%, 90.9%, 82.4% and 86.5% respectively within 5 days, and th...
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