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Artificial synthesis method and genetic analysis method of allotetraploid cotton

A genetic analysis method and an allotetraploid technology, applied in the field of cotton genetics and breeding, can solve problems such as high cost of use, failure to grow, and inability to promote high-quality cotton in large quantities, so as to improve efficiency, reduce the impact of external adversity, and avoid The effect of the risk of degradation

Inactive Publication Date: 2015-05-13
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, tetraploid cotton is limited by factors such as geography and climate, and cannot grow or reproduce in other regions, resulting in high-quality cotton that cannot be promoted in large quantities, and the cost of use is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: This embodiment provides a kind of synthetic method of allotetraploid cotton, and it comprises the following steps:

[0055] (1) Preparing donor materials: the donor materials include cotton seeds of Cotton chromosome group A and cotton seeds of Cotton chromosome D group;

[0056] (2) Carry out long-term uninterrupted lineal purification of donor materials under controlled climate conditions, and carry out single-boll multi-generation lineal purification breeding: the annual average temperature is 22.5-25.6°C, the temperature range is 10-35°C, and the annual sunshine hours 1780-2600 hours, total solar radiation 4500-5800 MJ / square meter, annual precipitation 1500-2500 mm, temperature-humidity index 65-70, photosynthetically active radiation 2100-2600MJ / m 2 Under the environmental conditions of the donor material, more than 3 generations of single-boll propagation are carried out within one year. After the growth and development and fruiting, the first boll...

Embodiment 2

[0103] Embodiment 2: This embodiment provides an artificial synthesis method of allotetraploid cotton and a genetic analysis method thereof, the steps of which are basically the same as in Example 1, the difference being:

[0104] The single bolls of the father and mother are harvested and replanted until 24 generations of high purification, and each highly purified plant is coded and listed for plant-to-plant hybridization and accurate genetic analysis. Two species of Cotton chromosome A group: Cotton grass and Asian cotton were used as female parents to hybridize with the wild species of Cotton chromosome D group respectively (see Table 1 for specific cotton species). Gently tap around the stigma to promote full contact between pollen grains and stigma, save identification time and reduce the impact of external adversity, and increase the rate of boll formation. On the second day after pollination, chemical insect-proof and anti-bacteria sprays were carried out, and then the...

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PUM

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Abstract

The invention discloses an artificial synthesis method for allotetraploid cotton. The method comprises steps as follows: (1), donor materials are prepared, wherein the donor materials comprise cotton seeds belonging to a gossypium chromosome group A and cotton seeds belonging to a gossypium chromosome group D; (2), the donor materials are constantly purified under a controlled climate condition for a long period of time; (3), the donor materials are subjected to fixed plant coding and perennial root storage; (4), the group-A donor materials and group-D donor materials obtained after multi-generation purification are subjected to distance hybridization; (5), a clear and stable male-parent specific band is taken as a judgment basis, hybridization generations are subjected to authenticity identification and genetic analysis; (6), the F1 generation which is identified to be true is subjected to gene mutation processing with an induced mutation method; hybridization plants or hybridization seeds obtained after screening are AD hybridization allotetraploid cotton. The invention further discloses a genetic analysis method of the allotetraploid cotton synthetized with the artificial synthesis method.

Description

technical field [0001] The invention relates to the field of cotton genetics and breeding, in particular to an artificial synthesis method of allotetraploid cotton under artificial control conditions and a genetic analysis method of the allotetraploid cotton. Background technique [0002] There are 51 species in the genus Gossypium, 46 of which are diploid cotton species (2n=2X=26), 5 are allotetraploid cotton species (2n=4X=52) and upland cotton (G .hirsutum, [AD]1), Sea Island cotton (G.barbadense, [AD]2), Hawaiian cotton (G.tomentosum, [AD]3), yellow brown cotton (G.mustillinum, [AD]4) and Darwin Cotton (G. darwinii, [AD] 5). [0003] For a long time, the issue of the donor species of the five allotetraploid species of Gossypium in nature has been a hotspot in the debate on the origin and evolution of Gossypium. Cotton allopolyploid has always been a good material for studying the evolution of repeated genes and repeated genomes in polyploids. In order to further explor...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H1/08
Inventor 陈弟张西岭李付广张海棠刘平
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI