A kind of acidophilic sulfate-reducing bacteria strain and its application
A sulfate and bacterial strain technology, applied in the field of microbial screening and application, can solve the problems of unknown bacterial structure, bacterial strains not resistant to acid, high cost, etc., and achieve the effect of overcoming uncertainty, good effect and vigorous metabolism
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Embodiment 1
[0041] Example 1 FKB strain isolation identification and functional analysis
[0042] 1) Strain screening
[0043] The sediment samples collected from the edge of the No. 1 tailings pond of Fankou Lead-Zinc Mine were collected in a sterile 50mL centrifuge tube, then placed in an ice bath, transported back to the laboratory, and immediately stored in a 4°C refrigerator.
[0044] After the sample is collected, enrichment culture is first carried out with a special enrichment medium, the purpose of which is to allow the sulfate-reducing bacteria that do not occupy a dominant position to multiply in large numbers and remove most of the heterotrophic bacteria. The culture medium for aSRB enrichment refers to the culture medium used by Sen and Johnson (1999) for cultivating sulfate-reducing bacteria, and corresponding improvements are made according to the actual situation. The composition of enrichment medium is: (NH 4 ) 2 SO 4 0.45g; KCl0.05g; MgSO 4 ·7H 2 O 0.5g; KH 2 PO ...
Embodiment 2F
[0051] The preparation of embodiment 2FKB liquid bacterial agent
[0052] 1) Inoculate the FKB bacteria into the enrichment medium for cultivation. Use a 100mL blue-capped glass bottle. After inoculation, blow nitrogen for 20 minutes to remove the air, and then place it in a 30°C incubator for static cultivation until the culture medium changes from clarification to ink color;
[0053] 2) Inoculate the cultured bacterial solution into a 10L seed tank according to the inoculum amount of 5%, blow nitrogen gas for 20 minutes, and cultivate at 30°C until the culture solution changes from clarification to ink color, and the liquid volume in the seed fermentation tank is 70%. The medium formula (w / v) is: sugarcane molasses 5%, yeast extract 2%, glucose 0.5%, (NH 4 ) 2 SO 4 4.5%, MgSO 4 ·7H 2 O 5%, KCl 0.5%, KH 2 PO 4 0.5%, Ca(NO 3 ) 2 4H 2 O 0.14%, FeSO 4 ·7H 2 O5%, ascorbic acid 0.5%, pH 5.2;
[0054] 3) Inoculate the fermented liquid in the seed fermented tank into th...
Embodiment 3F
[0056] Example 3 FKB is used for acidification control of dump site
[0057] The site is the southern dump of Jiangxi Yongping Copper Mine, with an area of 16,800 square meters, divided into two areas: the top and the side slope, and the side slope is 10,000m 2 , top 6800m 2 . This area has the following typical representative meanings:
[0058] (1) The slope is steep and the drop is large: According to the surveyed topographic map, the elevation of the top of the area is 273.3m, the elevation of the foot of the slope is 185.5m, the elevation difference reaches 87.8m, and the slope of the slope is between 1:1 and 1:1.25. It can basically represent the terrain of the dump site with steep slope and large drop.
[0059] (2) Severe erosion and piles of boulders: Due to years of erosion and erosion, more than 60% of the area’s slopes and slope toe are basically boulders without soil, and the living conditions for plants are extremely poor (no plants were seen at the site grow...
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