A preparation method of money turtle anti-tumor polypeptide
An anti-tumor and anti-tumor technology, which is applied in the field of preparation of anti-tumor polypeptides of money turtles, can solve the problems of large toxic side effects and unsatisfactory treatment effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] (1) Mix 5 g of minced turtle meat with ultrapure water and stir to prepare a mixed solution with a concentration of 10% (w / v). Add trypsin for enzymatic hydrolysis. The hydrolysis conditions are: PH=7, enzymatic hydrolysis temperature 35°C, enzymatic hydrolysis time 5 h, enzyme to substrate concentration ratio of 0.2% (w / w). In this process, 0.05 mol / L NaOH and HCl were used to adjust the pH value of the reaction system, and the pH value was controlled between pH ± 0.05. After the enzymolysis was completed, the enzyme was inactivated in 80°C water for 10 min, cooled to room temperature, and the enzymolysis The solution was centrifuged at 7000 r / min for 30 min, and the supernatant was collected.
[0027] (2) Take the enzymatic hydrolyzate obtained in step (1), and filter it successively with ultrafiltration membranes with molecular weight cut-offs of 10 KD, 5 KD and 3 KD (CO 2 The pressure is 0.1 Mpa at room temperature), so as to obtain the enzymatic hydrolyzate of mon...
Embodiment 2
[0031] (1) Mix 10 g of minced turtle meat with ultrapure water and stir to prepare a mixed solution with a concentration of 15% (w / v). Add trypsin for enzymatic hydrolysis. The hydrolysis conditions are: PH=8, enzymatic hydrolysis temperature 30°C, enzymatic hydrolysis time 10 h, enzyme to substrate concentration ratio of 0.1% (w / w). In this process, 0.1 mol / L NaOH and HCl were used to adjust the pH value of the reaction system, and the pH value was controlled between pH±0.05. After the enzymolysis was completed, the enzyme was inactivated in water at 95°C for 15 minutes, cooled to room temperature, and the enzymolysis The liquid was centrifuged at 6000 r / min for 35 min, and the supernatant was collected.
[0032] (2) Take the enzymatic hydrolyzate obtained in step (1), and filter it successively with ultrafiltration membranes with molecular weight cut-offs of 10 KD, 5 KD and 3 KD (CO 2 The pressure is 0.22 Mpa at room temperature), so as to obtain the enzymatic hydrolyzate o...
Embodiment 3
[0035] (1) Mix 18 g of minced turtle meat with ultrapure water and stir to prepare a mixed solution with a concentration of 30% (w / v). Add trypsin for enzymolysis. The hydrolysis conditions are: PH=9, enzymolysis temperature 60°C, enzymatic hydrolysis time 15 h, the ratio of enzyme to substrate concentration was 0.5% (w / w). In this process, 0.2 mol / L NaOH and HCl were used to adjust the pH value of the reaction system, and the pH value was controlled between pH±0.05. After the enzymolysis was completed, the enzyme was inactivated in 100°C water for 20 minutes, cooled to room temperature, and the enzymolysis The liquid was centrifuged at 8000 r / min for 60 min, and the supernatant was collected.
[0036] (2) Take the enzymatic hydrolyzate obtained in step (1), and filter it successively with ultrafiltration membranes with molecular weight cut-offs of 10 KD, 5 KD and 3 KD (CO 2 The pressure is 0.25 Mpa at room temperature), so as to obtain the enzymatic hydrolyzate of money turt...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More