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Golden camellia seedling propagation method

A technology for golden camellia tea and seedlings, applied in the field of plant tissue culture, can solve the problems such as the research on the differentiation of callus into seedlings without a comprehensive and systematic report, and achieve the effects of improving the germination rate, speeding up the proliferation rate and reducing consumption.

Active Publication Date: 2015-05-27
FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In addition, there are reports on the use of seeds, daughter embryos, leaves, etc. as explants, but this study is limited to the induction of callus, and there is no comprehensive and systematic report on the differentiation of callus into shoots

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of golden camellia seedling propagation method, operates according to the following steps:

[0029] (1) Fruit pretreatment

[0030] Select 7-8 mature, uncracked, green, plump, and pest-free fruits of Camellia japonica native to Fangchenggang City, Guangxi. Use a brush to clean impurities on the surface of the fruit, then soak in saturated detergent water for 20 minutes, and rinse with running water for 30 minutes;

[0031] (2) Sterilization treatment

[0032] Soak in 75% alcohol for 15 minutes on the ultra-clean workbench, wash with sterile water for 3 times, then cut open the fruit with a scalpel, take out the seeds, soak in 0.1% mercury liter for 7 minutes, wash with sterile water for 4 times, and finally inoculate In the plate, cut the seed coat, take out the endosperm, insert it into the induction medium, and place the end with the germ at the bottom of the medium, and the germ part of the seed should not be damaged during the operation;

[0033] (3) Germin...

Embodiment 2

[0049] A kind of golden camellia seedling propagation method, operates according to the following steps:

[0050] (1) Fruit pretreatment

[0051] Select 7-8 mature, uncracked, green, plump, and pest-free fruits of Camellia japonica native to Fangchenggang City, Guangxi. Use a brush to clean impurities on the surface of the fruit, then soak in saturated detergent water for 15 minutes, and rinse with running water for 20 minutes;

[0052] (2) Sterilization treatment

[0053] Soak in 75% alcohol for 20 minutes on the ultra-clean workbench, wash with sterile water 4 times, then cut open the fruit with a scalpel, take out the seeds, soak in 0.1% mercury liter for 5 minutes, wash with sterile water 4 times, and finally inoculate In the plate, cut the seed coat, take out the endosperm, insert it into the induction medium, and place the end with the germ at the bottom of the medium, and the germ part of the seed should not be damaged during the operation;

[0054] (3) Germination in...

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PUM

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Abstract

The invention discloses a golden camellia seedling propagation method. The method comprises the following steps: pretreating fruits, sterilizing the fruits, performing germinating induction, performing initial subculture, performing mulitiplication, strengthening the seedlings, hardening the seedlings, rooting outside the bottle and performing integrated management. The method disclosed by the invention has the advantages of the capacity of rapidly obtaining lots of aseptic seedlings, simplicity in operation, low pollution rate and high germination rate.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a seedling breeding method of camellia japonica. Background technique [0002] Since the development of Camellia japonica tissue culture rapid propagation technology, there are few research reports on in vitro culture of Camellia japonica, and the research mainly focuses on the use of adult plant stems as explants to induce seedlings, such as Zheng Ruoxian (1980), Liao Hanren (1987 ), Yan Muqin (1988), Lin Li (2005). However, there are still many problems in using stems as explants. For example, it is difficult to sterilize the stems thoroughly and it is easy to get moldy; the stems of woody plants are easy to brown. Bacteria time treatment can alleviate browning to a certain extent, but the effect is not ideal; on the other hand, it is difficult to induce axillary buds, and it is not easy to master. Camellia japonica grows slowly, and the branches are relatively p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄昌艳周主贵卜朝阳卢家仕何荆洲闫海霞王晓国
Owner FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI