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Mutagenesis of RNA-rich Saccharomyces cerevisiae

A ribonucleic acid and Saccharomyces cerevisiae strain technology is applied in the field of mutagenesis of ribonucleic acid-rich Saccharomyces cerevisiae, and can solve the problems of not increasing RNA content and the like

Active Publication Date: 2017-12-15
唐山拓普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the breeding of brewer's yeast, there is no research report on increasing the RNA content through mutagenesis screening of the atmospheric pressure and room temperature plasma mutagenesis system

Method used

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  • Mutagenesis of RNA-rich Saccharomyces cerevisiae
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  • Mutagenesis of RNA-rich Saccharomyces cerevisiae

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Experimental program
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Embodiment 1

[0034] Brewer's yeast was selected as the starting strain, and its morphological characteristics and culture characteristics were as follows: the cells were oval, and the aspect ratio of the cells was between 1 and 2;

[0035] The colony shape is milky white, perfectly round, with neat edges, shiny and flat;

[0036] Culture characteristics: the optimum growth temperature is 28°C, the optimum growth pH is 6.5-7.0, and the shaker speed is 150-200r / min. When cultured for 20 hours, the RNA content is the highest, which is 6.78%-7.06%.

[0037] Brewer's yeast strain QH641 was screened for high ribonucleic acid by atmospheric pressure and room temperature plasma mutagenesis.

[0038] Inoculate brewer's yeast strains that grow well and conform to the above-mentioned morphological characteristics in malt extract powder broth medium, shake culture at 28°C for 24h, and make 10 7 cells / mL of bacterial suspension.

[0039] Use a pipette gun to pipette 10 μL of the above bacteria suspen...

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Abstract

The invention provides an induced mutation method of ribonucleic acid-enriched saccharomyces cerevisiae. The induced mutation method comprises the following steps: culturing a saccharomyces cerevisiae strain to obtain a strain suspension; preprocessing the strain suspension, treating in a normal-pressure, room-temperature plasma induced mutation system, and screening out a ribonucleic acid-enriched saccharomyces cerevisiae variant strain, thereby obtaining QH641; performing fermentation cultivation on the QH641 to obtain QH641 bacteria high in ribonucleic acid content. The induced mutation method of the ribonucleic acid-enriched saccharomyces cerevisiae has the advantages of high genetic stability and high RNA content enrichment ability of the strain, and after fermentation, the maximum RNA content can be 15%-17% of content of the bacteria.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a mutagenesis method for ribonucleic acid-rich brewer's yeast. Background technique [0002] Ribonucleic acid (RNA) is ubiquitous in animal, plant and microbial cells and participates in physiological activities such as cellular protein synthesis and immune regulation. In view of the fact that the products obtained by microbial fermentation are safe, non-toxic, short production cycle, and cost-effective, which are superior to other methods, microbial-derived RNA and its degradation product nucleotide products are used in food, health care products, medicine, agriculture and other related fields. The outlook is getting better and better. In addition, autolysis, enzymolysis, filtration and concentration of yeast cells to obtain yeast extracts rich in natural flavor nucleotides (I+G) have gradually become a research and development hotspot for modern yeast deep-processing enterprises. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/01C12R1/865
Inventor 陈志颖张子健江建梅舒媛吴振
Owner 唐山拓普生物科技有限公司