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Method for enriching tanshinol in fermentation broth by using macroporous resin

A technology of pore adsorption and danshensu, which is applied in the field of separation and purification of fermentation products, can solve the problems that the method of danshensu has not been published in the newspapers, etc., and achieve good industrial application prospects, high yield and simple operation

Inactive Publication Date: 2015-06-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of separating and purifying Danshensu from E. coli fermentation broth has not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for enriching danshensu in fermentation broth by using macroporous adsorption resin includes the following steps:

[0027] (1) Centrifuge the fermentation broth obtained by fermenting the Danshensu-producing Escherichia coli to obtain the supernatant, adjust the pH to 2.0, and use it as the loading solution for use;

[0028] (2) Accurately weigh 50g of pretreated macroporous adsorption resins of X-5 and ADS-17, respectively, wet pack the column (25*300 with sand core), respectively, the sample flow rate is 2BV / h and 250mL step (1) The obtained loading solution is added to the macroporous adsorption resin column;

[0029] (3) Add 50 mL of 80% ethanol aqueous solution to the macroporous adsorption resin column, elution through the macroporous adsorption resin column at a flow rate of 1 BV / h, and collect 10-40 mL of the eluate;

[0030] (4) Concentrate and dry the eluate to obtain Danshensu.

[0031] Calculate adsorption capacity, elution volume and elution rate.

[0032] T...

Embodiment 2

[0035] A method for enriching danshensu in fermentation broth by using macroporous adsorption resin includes the following steps:

[0036] (1) Centrifuge the fermentation broth obtained by fermenting the Danshensu-producing Escherichia coli to obtain the supernatant, take 3 aliquots and adjust the pH=1.5, pH=2.0, and pH=3.0 respectively, as the loading solution, for use;

[0037] (2) Accurately weigh 3 pretreatment models of X-5 macroporous adsorption resin each with 50g, and wet column (25*300 with sand core) respectively to 250mL pH=1.5, pH=2.0, pH=3.0 The loading liquid is added to the X-5 macroporous adsorption resin column at a flow rate of 2BV / h;

[0038] (3) Add 50 mL of 80% ethanol aqueous solution to the macroporous adsorption resin column, elution through the macroporous adsorption resin column at a flow rate of 1 BV / h, and collect 10-40 mL of the eluate;

[0039] (4) Concentrate and dry the eluate to obtain Danshensu.

[0040] The calculated adsorption capacity is 6.325mg / g,...

Embodiment 3

[0042] A method for enriching danshensu in fermentation broth by using macroporous adsorption resin includes the following steps:

[0043] (1) Centrifuge the fermentation broth obtained by fermenting the Danshensu-producing Escherichia coli to obtain the supernatant, adjust the pH to 2.0, and use it as the loading solution for use;

[0044] (2) Accurately weigh 3 pretreatment models of X-5 macroporous adsorption resin, each with 50g, and wet pack the column (25*300 with sand core) respectively take 150mL, 200mL, 250mL of the loading liquid, and the above sample flow rate Add 2BV / h to X-5 macroporous adsorption resin column;

[0045] (3) Add 50 mL of 80% ethanol aqueous solution to the macroporous adsorption resin column, elution through the macroporous adsorption resin column at a flow rate of 1 BV / h, and collect 10-40 mL of the eluate;

[0046] (4) Concentrate and dry the eluate to obtain Danshensu.

[0047] The calculated adsorption capacity is 4.47mg / g, 5.674, 6.646mg / g.

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Abstract

The invention discloses a method for enriching tanshinol in fermentation broth by using macroporous resin. The method comprises the following steps: (1) centrifuging fermentation broth obtained by fermenting Escherichia coli SyBE 002444 with a preservation number of CGMCC No.7961 to obtain a supernatant, and regulating the pH to 1.5-3.0 to serve as a liquid sample for later use; (2) adding the liquid sample into a macroporous resin column; (3) adding an ethanol aqueous solution with volume concentration of 70-90% into the macroporous resin column, eluting the liquid sample at a flow rate of 0.5-2 BV / H through the macroporous resin column, and collecting eluant; (4) concentrating and drying the eluant to obtain tanshinol. The method disclosed by the invention has the advantages of simplicity in operation, high yield, environmental friendliness and the like. The method disclosed by the invention can be applied to separation and purification of tanshinol production by microbiological fermentation.

Description

Technical field [0001] The invention relates to the separation and purification of fermentation products, in particular to a method for enriching danshensu in fermentation broth by using a macroporous adsorption resin. Background technique [0002] Macroporous adsorption resin is a kind of organic polymer copolymer with macroporous structure, and it is a kind of synthetic organic polymer adsorbent. Macroporous resin adsorption technology was first used in wastewater treatment, pharmaceutical industry, chemical industry, analytical chemistry, clinical verification and treatment, etc. In recent years, it has been widely used in the extraction, separation and purification of active ingredients of Chinese herbal medicines, such as flavonoids and saponin , Refining and purification of alkaloids and other traditional Chinese medicine ingredients. [0003] Danshensu (Salvianic acid A, Danshensu) is an acidic aromatic compound, its chemical name is β-(3,4-dihydroxyphenyl) lactic acid, als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C59/52C07C51/47
CPCC07C51/47C07C59/52
Inventor 赵广荣白晨龙
Owner TIANJIN UNIV