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A method for preparing hemp fiber by combining Candida tropicalis dk2 strain and hydrogen peroxide ultrasonic wave

A joint preparation technology of Candida tropicalis, applied in the direction of ultrasonic/sonic fiber treatment, microbial-based methods, biochemical equipment and methods, etc., can solve the problem of not being able to meet the needs of high-grade and high-count fabrics, degumming quality affecting fabric appearance, The degumming process takes a long time to achieve the effect of strong anti-pollution ability, improved degumming effect and low processing cost

Inactive Publication Date: 2016-09-07
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The chemical degumming method commonly used in the industry needs to be washed 4-6 times, and the production of one ton of dry hemp usually requires 500-700 tons of water, and the work is discontinuous in production, the labor intensity is high, the degumming process takes a long time, and the energy consumption is high. Quality Affects Fabric Appearance
It cannot meet the needs of high-grade and high-count fabrics

Method used

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  • A method for preparing hemp fiber by combining Candida tropicalis dk2 strain and hydrogen peroxide ultrasonic wave
  • A method for preparing hemp fiber by combining Candida tropicalis dk2 strain and hydrogen peroxide ultrasonic wave
  • A method for preparing hemp fiber by combining Candida tropicalis dk2 strain and hydrogen peroxide ultrasonic wave

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Experimental program
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Effect test

Embodiment 1

[0028] This embodiment records the method for preparing degumming enzyme liquid from Candida tropicalis DK2:

[0029] (1) Store the Candida tropicalis DK2 strain in PDA medium, culture it at 200 rpm at 30°C for 24 hours, add freezing buffer; Into a 300mL Erlenmeyer flask, insert the obtained bacteria loop, and culture in a shaker at 150rpm at 28°C for 3 days, then culture the 50ml shaker flask, inoculate the culture medium into 5L of flax fermentation medium, and inoculate at a speed of 200r / min , Shake flask culture at a temperature of 30°C for 24 hours to obtain a fermentation broth. The fermentation broth was filtered through sterile gauze, the mycelium was washed with sterile water and dispersed in a Waring high-speed crushing cup for 10 seconds, and collected in sterile water. (with the same volume as the fermentation broth) to make a suspension of mycelium, and store it at 40°C for future use.

[0030] The formula of freezing buffer per 100ml is: potassium dihydrogen ph...

Embodiment 2

[0036] The process of preparing laccase from Candida tropicalis DK2 strain combined with hydrogen peroxide ultrasonic wave for degumming flax raw stems is as follows:

[0037] Put 5g of flax raw stems in a 250ml Erlenmeyer flask, fill the flask with a mixture of flax stems, crude enzyme solution and hydrogen peroxide at a ratio of 1:20, the liquid volume is 100ml, the pH value of the mixture is 6.0, flax The mass ratio of the original stem to the hydrogen peroxide is 1:5, and the mass ratio to the crude enzyme solution is 1:15. Degumming at 40°C and 200rpm shaker for 1 day, 2 days and 4 days respectively, the ultrasonic power is 80%, and 10% sodium hydroxide is added after the set time, the set temperature is 75°C, and the heating rate is 1°C / min , the time is 5 hours, the ultrasonic power is 20%, the degumming time is up, remove the degumming liquid, rinse several times with tap water, to remove the bacteria and non-cellulose substances on the original flax stem, and complete...

Embodiment 3

[0040] The process of preparing laccase from Candida tropicalis DK2 strain combined with hydrogen peroxide ultrasonic wave for ramie yarn is as follows:

[0041] Put 5g of ramie sterilized yarn (ramie roving or ramie spun yarn) into a 250ml conical flask, and divide the mixture of ramie yarn, crude enzyme solution and hydrogen peroxide into the conical flask at a ratio of 1:20, and the liquid volume is 100ml , the pH value of the mixed solution is 7.0, the mass ratio of ramie yarn to hydrogen peroxide is 1:1, and the mass ratio to crude enzyme solution is 1:19. Degumming at 10°C, 10rpm shaker for 10min, ultrasonic power 10%, add 0.5% sodium hydroxide after the set time, set temperature 50°C, heating rate 10°C / min, time 10min, ultrasonic power When the degumming time is up, remove the degumming liquid, rinse several times with tap water, to remove bacteria and non-cellulose substances on the ramie yarn, and complete degumming.

[0042] The observation of ramie yarn degumming f...

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Abstract

The invention relates to a method for preparing fibrilia by combination of candida tropicalis DK2 strains and hydraulic peroxide ultrasonic wave. The method comprises the following steps: (1) culturing the strains; (2) preparing crude enzyme; (3) mixing sterilized bast raw material and a mixed solution of crude enzyme and hydraulic peroxide in mass volume ratio of 1 to (10-30), regulating the pH, processing by shaking table under an ultrasonic condition, then adding sodium hydroxide, heating to remove glue, removing glue removing liquid, and flushing until pH reaches the natural value so as to finish glue removal. According to the method, the strain growth period is shortened, the strains are difficultly polluted, the treatment cost is low, and the glue removing conditions are mild; according to the glue removing method, the process is simple; the glue removing time is shortened; the energy consumption is reduced; the pollutant emission is reduced, so that the environment can be protected.

Description

technical field [0001] The invention belongs to the technical field of biological textiles, and in particular relates to a method for preparing hemp fiber by combining Candida tropicalis DK2 strain and hydrogen peroxide ultrasonic waves. Background technique [0002] Hemp is one of the raw materials for textiles, and its varieties mainly include flax, ramie, hemp, kenaf, jute, apocynum, sisal, abaca, nettle and velvetle. Ramie is a unique hemp resource in my country, known as "Chinese grass". my country is the main producer of ramie in the world, and its output accounts for more than 90% of the world's total output; Textile fibers made of fabrics are widely used. Hemp fiber is also one of the earliest textile fibers used by humans, and my country's hemp output ranks first in the world. As a green ecological fiber, hemp fiber has excellent properties such as moisture absorption, breathability, antibacterial, anti-mildew, anti-ultraviolet, and no static electricity. Driven b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01C1/04D06M10/02C12N9/02C12R1/74
CPCD01C1/04
Inventor 丁若垚刘国亮郁崇文张兴群
Owner DONGHUA UNIV
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