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A highly sensitive and highly selective method for detecting benzo[a]pyrene

A high-selectivity, benzo-based technology, applied in color/spectral characteristic measurement, measuring devices, instruments, etc., can solve problems such as difficult high sensitivity, sensitivity dependent on detector sensitivity, unsuitable rapid detection, etc., to improve sensitivity and selection The effect of sex and enrichment efficiency

Active Publication Date: 2018-03-27
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of benzo[a]pyrene in the environment is very low, and the commonly used detection methods such as fluorescence spectroscopy and liquid chromatography cannot meet the detection requirements
The detection limit of benzo[a]pyrene by fluorescence spectroscopy is at the μg / L level, and it will be interfered by other fluorescent substances and fluorescence quenching substances in the sample; although liquid chromatography has good selectivity, its Sensitivity depends on the sensitivity of the detector, and the current conventional detectors can only reach the detection limit of μg / L level
In addition, fluorescence spectroscopy and liquid chromatography instruments are complex, and it is difficult to ensure high sensitivity while being miniaturized. Complicated sample concentration and pretreatment processes are required before detection, and the detection time is long, which is not suitable for rapid on-site detection.

Method used

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  • A highly sensitive and highly selective method for detecting benzo[a]pyrene
  • A highly sensitive and highly selective method for detecting benzo[a]pyrene
  • A highly sensitive and highly selective method for detecting benzo[a]pyrene

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Embodiment 1

[0018] In the present invention, 10 -10 The detection of mol / L benzo[a]pyrene is taken as an example. The operation steps are as follows:

[0019] (1) Preparation of hydrophilic benzo[a]pyrene molecularly imprinted solid-phase extraction packing and solid-phase extraction column

[0020] Dissolve 1 mM benzo[a]pyrene and 1 mM 4-vinylpyridine in 30 mL of dichloromethane solution, and place in the dark for 1 hour. Add 5 mM glycidyl methacrylate, 5 mM divinylbenzene, and 1 mM azobisisobutyronitrile, and dissolve them by ultrasonication. Nitrogen gas was passed through the resulting solution for 10 minutes, sealed, and reacted in a constant temperature oil bath at 70°C for 24 hours. The obtained polymer was pulverized and ground, and the obtained polymer was placed in a filter paper tube, and dichloromethane was used as an eluent, and the template molecule phenanthrene was removed by Soxhlet extraction for 48 hours. Air-dry the obtained imprinted polymer at room temperature to ...

Embodiment 2

[0037] In the present invention, 5×10 -10The detection of mol / L benzo[a]pyrene is taken as an example. The difference between its operation steps and that of Example 1 is that the absorbance value of the 1ml methanol solution of benzo[a]pyrene in the water body under separation and enrichment elution using the obtained solid-phase extraction column is detected by using a spectral detection system, according to the standard work The curve calculates the concentration of benzo[a]pyrene to be 4.86×10 - 10 mol / L.

Embodiment 3

[0039] In the present invention, 10 -9 The detection of mol / L benzo[a]pyrene is taken as an example. The difference between its operation steps and that of Example 1 is that the absorbance value of the 1ml methanol solution of benzo[a]pyrene in the water body under separation and enrichment elution using the obtained solid-phase extraction column is detected by using a spectral detection system, according to the standard work The curve calculates the concentration of benzo[a]pyrene to be 9.55×10 -8 mol / L.

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Abstract

The invention belongs to the technical field of analysis and testing of polycyclic aromatic hydrocarbons, in particular to a method for detecting benzo[a]pyrene with high sensitivity and high selectivity. Specifically, the sample is separated and enriched in a molecularly imprinted solid phase column with a hydrophilic functional group molecularly imprinted solid phase extraction filler, and then the liquid core waveguide detection technology is used to quantitatively detect the above enriched sample liquid at 385nm. of benzo[a]pyrene. Using the method of the invention can realize the rapid detection of benzo[a]pyrene in environmental water with high sensitivity and high selectivity.

Description

technical field [0001] The invention belongs to the technical field of analysis and testing of polycyclic aromatic hydrocarbons, in particular to a method for detecting benzo[a]pyrene with high sensitivity and high selectivity. Background technique [0002] Polycyclic aromatic hydrocarbons are a class of organic compounds containing two or more benzene rings or heterocycles. As a typical class of persistent organic pollutants, polycyclic aromatic hydrocarbons widely exist in water, air, soil, organisms and other environments. The physical and chemical properties of polycyclic aromatic hydrocarbons are stable, difficult to degrade, easy to accumulate in the human body and organisms, have strong carcinogenic, teratogenic, and mutagenic effects, and cause great harm to human health and marine life. Among these polycyclic aromatic hydrocarbons, the most representative one is benzo[a]pyrene, which widely exists in coal tar, various types of carbon black, and flue gas, cigarette s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N30/08
Inventor 秦伟李龙魏文超
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI