Unlock instant, AI-driven research and patent intelligence for your innovation.

A microfluidic chip for screening antitumor drugs at the single sphere level and its application

An anti-tumor drug, microfluidic chip technology, applied in laboratory containers, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of high price and complicated operation steps, and achieve simple operation , environment-friendly, and low experimental cost

Active Publication Date: 2016-09-07
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a microfluidic chip for screening anti-tumor drugs at the single-sphere level and its application, so as to solve the cumbersome and complicated operation steps and high price in the previous production process, and the rapid formation of tumor microspheres and their anti-tumor drugs. High-throughput and high-content drug screening

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A microfluidic chip for screening antitumor drugs at the single sphere level and its application
  • A microfluidic chip for screening antitumor drugs at the single sphere level and its application
  • A microfluidic chip for screening antitumor drugs at the single sphere level and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The SU-8 polymer template is made by photolithography technology, and the template is 12 groups of parallel microholes with 7 different diameters, the diameters are 200, 300, 400, 500, 600, 700, and 800 microns. 6 groups of the same size in parallel. Use conventional photoetching technology to make SU-8 template: apply SU-8 photoresist on a clean glass sheet, bake it at 95 degrees Celsius for 1 hour, remove the organic reagent, and wait for the SU-8 photoresist to cool ; Put the designed mask on and expose to UV for 90 seconds, then bake at 95 degrees Celsius for 15 minutes. Different from the conventional method, the development time of the SU-8 template of the present invention is 5 minutes, which is incomplete development. After drying the developer, it is melted at 85°C for 5 minutes, so that the structure of the micropores in the SU-8 template is changed from The right angle becomes an arc; after cooling down, the SU-8 template is fully exposed to the ultraviolet ...

Embodiment 2

[0041] Microsphere culture of U87 cells on a sealed PDMS polymer chip such as Figure 4 shown. The PDMS polymer chip was sterilized first, irradiated with ultraviolet light overnight, and then used 0.2% PF-127 solution for surface modification for 4 hours, washed twice with sterile water and PBS solution, and inoculated with U87 cell suspension. Under the action of gravity, it will settle to the bottom of the small hole on the surface of the chip and spontaneously form a three-dimensional cell sphere. As a result of designing microwells of different sizes, U87 cells formed multicellular microspheres of different sizes.

Embodiment 3

[0043] Screening of anti-tumor drugs at the single-ball level Microfluidic chips are used for the screening of anti-cancer drugs in U87 cells. Figure 5 It shows that after U87 cells were cultured for four days on PDMS polymer chips with concave holes of different sizes, 200 μmol / L resveratrol drug was added through the upper flow channel layer to act on U87 cell microspheres, and some multicellular microspheres The diameter of the cells became smaller, and the cell viability decreased, but the cell apoptosis did not appear. Figure 6 It shows that after U87 cells were cultured on PDMS polymer chips with different sizes of concave holes for four days, 1.5 μmol / L PI-103 drug was added through the upper flow layer, the diameter of some multicellular microspheres became smaller, and the cell viability decreased, cell apoptosis occurred.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A micro-fluidic chip used for antitumor drug screening at the single-ball level and applications are disclosed. A method includes a step of manufacturing a PDMS channel chip template by utilizing a hot-melt SU-8 template. Sunken holes of a plurality of sizes can be manufactured by a one-step method and used for forming tumor microballs with different sizes. The micro-fluidic chip is a chip comprising two layers, comprises twelve parallel micro-fluidic units, and can be used for antitumor drug screening at the single-ball level for tumor cells. The method is free of expensive etching devices, and has advantages of simple operation, rapidness, low experiment cost, no organic solvents, and capability of being environmental friendly and being integrated with other techniques.

Description

technical field [0001] The invention belongs to the field of processing, manufacturing, modification and application of polymer chips, and in particular relates to a microfluidic chip for three-dimensional single-ball level antitumor drug screening and its application. Background technique [0002] Monolayer cell culture is the main method of in vitro cell culture. It has the advantages of simple culture, easy operation, low cost, and large-scale application. It is widely used as an experimental model for in vitro pharmacology and toxicology research. However, the conventional in vitro monolayer culture method cannot provide the environmental conditions required for the normal growth and development of tissues, and the growth environment has an important impact on cell gene expression and behavior. The genome of each cell in the body is the same, but different organs and tissues have the same The phenotype and functional structure are different. For example, ectopic transpla...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00C12Q1/02
Inventor 秦建华石杨李艳峰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI