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High spatial resolution laser differential confocal mass spectrometry microscopy imaging method and device

A high spatial resolution, differential confocal technology, applied in the field of confocal microscopy imaging technology and mass spectrometry imaging, can solve the problems of low spatial resolution of mass spectrometry detection, large laser focus spot, long time of mass spectrometry imaging, etc., to achieve improved spatial resolution ability, the effect of suppressing drift

Active Publication Date: 2017-07-07
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1) Due to the use of simple laser focusing to desorb and ionize the sample, there are still problems such as large laser focusing spot and low spatial resolution of mass spectrometry detection;
[0007] 2) The time required for mass spectrometry imaging is long, and the axial position of the focused spot of the laser mass spectrometer often drifts relative to the sample to be measured

Method used

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  • High spatial resolution laser differential confocal mass spectrometry microscopy imaging method and device
  • High spatial resolution laser differential confocal mass spectrometry microscopy imaging method and device
  • High spatial resolution laser differential confocal mass spectrometry microscopy imaging method and device

Examples

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Embodiment 1

[0048] The embodiment of the present invention is based on image 3 and Figure 4 The shown high spatial resolution laser differential confocal mass spectrometer imaging device includes a laser point light source system 24 composed of a pulse laser 26, a focusing lens 27 and a pinhole 28 located at the focal point of the focusing lens 27, which are placed in sequence along the optical axis The collimating lens 25, the outgoing beam attenuator 34, the ring light generating system 3, the beam splitter 5, the center hole reflector 6 and the focusing center hole reflector 6 placed along the direction of the refracted optical axis reflect the light beam to the measured sample 8 The middle hole measurement objective lens 7 also includes a detection beam attenuator 35 and a differential confocal light intensity detector 2 for detecting the reflected light intensity signal of the focused light spot of the middle hole measurement objective lens 7, and is used for detecting the focused ...

Embodiment 2

[0068] like Figure 5 As shown, in the high spatial resolution laser differential confocal mass spectrometry microscopic imaging device of embodiment 1, the ring light generating system 3 is replaced by a vector beam generating system 21 and a pupil filter 22 placed along the optical axis direction to generate a vector beam , a ring-shaped beam 23 is generated, which is focused to a tiny light spot exceeding the diffraction limit after passing through the center-hole reflector 6 and the center-hole measuring objective lens 7 and irradiates on the measured sample 8 .

[0069] The remaining imaging measurement methods are the same as in Example 1.

Embodiment 3

[0071] like Figure 5 As shown, in the high-spatial-resolution laser confocal mass spectrometer imaging device of Embodiment 1, the computer 10 can control the two-dimensional scanning galvanometer system 33 so that the middle hole measurement objective lens 7 is aligned with the next area to be measured of the sample 8 to be measured .

[0072] The remaining imaging measurement methods are the same as in Example 1.

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Abstract

The invention relates to a high spatial resolution laser differential confocal mass spectrometry microscopic imaging method and device, belonging to the technical fields of confocal microscopic imaging technology and mass spectrometry imaging. The present invention combines differential confocal imaging technology, mass spectrometry imaging technology and spectral detection technology, utilizes the focusing spot of high spatial resolution differential confocal The same focused spot of the focal microscope system desorbs and ionizes the sample for mass spectrometry imaging, and then realizes high spatial resolution imaging of sample micro-region images and components. The device includes a point light source, a collimator lens, a ring light generating system, a beam splitter, a center hole mirror and a center hole measurement objective lens, and also includes a differential confocal intensity detector for detecting the reflected light intensity signal of the focused spot, and for Ionization sample pipette and mass detection system to detect plasma plume components. The invention can be used for high-resolution imaging of biological mass spectrum.

Description

technical field [0001] The invention belongs to the field of confocal microscopic imaging technology and mass spectrometry imaging technology, combines laser differential confocal microscopic imaging technology and mass spectrometric imaging technology, and relates to a high spatial resolution laser differential confocal mass spectroscopic microscopic imaging method and device, which can be used in biological High-resolution imaging of mass spectrometry. [0002] technical background [0003] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on multiple small areas in the two-dimensional area of ​​the sample to detect th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/71G01N27/64
Inventor 赵维谦邱丽荣
Owner BEIJING INSTITUTE OF TECHNOLOGYGY