Biomarker detection method and system based on surface plasmon resonance technology

Abstract

Surface plasmon resonance technology-based method, system, and apparatus for biomarker testing. The testing method is such that a beam emitted via a sensor chip is received by an optical receiver module to form a spectrum, the spectrum information is adjusted, and change information corresponding to a zero position is correspondingly acquired, thus the concentration of a biomarker of a test substance is acquired. The system comprises the optical receiver module (12), a control module (13), a spectral position adjusting apparatus (14), and a power supply module (15) and is used for implementing the method. The apparatus has the transmission mechanism driven into motion by the coil (9) into which an electric current can be introduced to adjust the orientation of a mirror. The beam emitted from the sensor chip is reflected by the mirror and then shone onto the optical receiver module. The method allows convenient and rapid changes to the spectral position of an emitted light of a biomarker in a test substance, thus acquiring validity information of the biomarker in the test substance.

Description

technical field [0001] The present invention relates to the field of biomarker detection, more specifically to a biomarker detection method and system based on surface plasmon resonance technology. Background technique [0002] Biomarkers refer to biochemical indicators that can mark changes or possible changes in system, organ, tissue, cell and subcellular structure or function, and have a very wide range of uses. Biomarkers can be used for disease diagnosis, judgment of disease stage, or to evaluate the safety and effectiveness of new drugs or new treatments in the target population. When detecting biomarkers, surface plasmon resonance technology SPR (Surface Plasmon Resonance) is commonly used, which uses a physical optical phenomenon caused by the total reflection connection of metal film / liquid surface interface light to analyze biomolecular interactions, and through infrared Light combined with SPR technology to measure the concentration of biomarkers. For details, pl...

AI Technical Summary

Problems solved by technology

[0003] In the field of biomarker detection, photometry is a commonly used method. It mixes the body fluid samples of the human body with one or more test reagents in vitro to determine the concentration of the analyte multiple times, thereby triggering biological A chemical reaction that causes a measurable change in the optical properties of the analyte. Photometry detects and utilizes the reduction in light flux that occurs when passing through an absorbing and / or astigmatic medium. However, this measurement method is due to Affected by the type of the sample itself and the interfering substances it may contain, system errors are prone to occur during measurement, making the measurement results of the concentration of biomarkers inaccurate, and it is difficult to control the adjustment of the light flow direction during measurement , which often brings great trouble to the measurer

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