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Rapid breeding method of anoectochilus zhejiangensis Z.Wei&Y.B.Chang seedlings

A technology of golden line orchid seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of endangered resources, shortage of seedlings, etc., achieve fast reproduction speed, enhance growth potential, and improve cultivation survival rate Effect

Active Publication Date: 2015-08-12
福建省农业科学院农业生物资源研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of rapid propagation method of Zhejiang golden line orchid seedlings, to overcome the existing method of rapid propagation of its seedlings not yet established, to solve the problems of its seedling shortage and endangered resources, the method provided by the present invention It can not only effectively improve the reproductive efficiency of Zhejiang golden line orchid, but also prevent the excessive growth and vitrification of test tube seedlings, so as to obtain a large number of Zhejiang golden thread blue seedlings, laying the foundation for its large-scale production and application

Method used

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Embodiment 1

[0051] Example 1 (best example)

[0052] The method for rapid propagation of Zhejiang golden thread orchid seedlings, the method includes the following steps in sequence:

[0053] (1) Preparation stage of explant

[0054] Collect wild Zhejiang golden thread orchid plants, plant them in the clean river sand of 25 mesh net indoor, and cultivate them for 15 days; select the robust plants that start to grow again, the leaves are stiff, the stem nodes are stretched, and the ground is cut. Part of the young stem nodes, with leaves removed, are used as explants; on an ultra-clean workbench, disinfect the explants with 10% by mass sodium hypochlorite solution for 10 minutes, and rinse with sterile water 3 times ; Each rinse time is 2 minutes; drain the water for later use;

[0055] (2) Primary induction culture stage

[0056] Under aseptic conditions, cut the explant aseptically processed in step (1) into a stem segment with a stem node, the length of the stem segment is 1.0 cm, and the lengt...

Embodiment 2

[0064] The method for rapid propagation of Zhejiang golden thread orchid seedlings, the method includes the following steps in sequence:

[0065] (1) Preparation stage of explant

[0066] Collect cultivated Zhejiang golden thread orchid plants, plant them in clean river sand in a 20-mesh net room, and cultivate them for 16 days; select the robust plants that have resumed growth, stiff leaves and stretched stem nodes, and cut them. The young stem nodes of the above-ground part, the leaves are removed, as explants; on an ultra-clean workbench, the explants are sterilized with a 9% mass percent sodium hypochlorite solution for 8 minutes, and rinsed with sterile water 3 Times; each rinse time is 1 minute; drain the water for later use;

[0067] (2) Primary induction culture stage

[0068] Under aseptic conditions, cut the explant aseptically processed in step (1) into a stem segment with a stem segment, the length of the stem segment is 0.9cm, and the length of the morphological lower en...

Embodiment 3

[0076] The method for rapid propagation of Zhejiang golden thread orchid seedlings, the method includes the following steps in sequence:

[0077] (1) Preparation stage of explant

[0078] Collect wild Zhejiang golden thread orchid plants, plant them in clean river sand with a mesh of 40 mesh indoors, and cultivate them for 20 days; select the robust plants that have resumed growth, stiff leaves and stretched stem nodes, and cut the ground. Part of the young stem nodes, with the leaves removed, are used as explants; on an ultra-clean workbench, use a sodium hypochlorite solution with a mass percentage concentration of 11% to disinfect the explants for 12 minutes and rinse with sterile water. 5 times; each rinse time is 2 minutes; drain the water for later use;

[0079] (2) Primary induction culture stage

[0080] Under aseptic conditions, cut the explant aseptically processed in step (1) into a stem segment with a stem node, the length of the stem segment is 1.1 cm, and the length of ...

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Abstract

The invention relates to a rapid breeding method of anoectochilus zhejiangensis Z.Wei&Y.B.Chang seedlings, wherein the method can effectively improve the breeding efficiency of anoectochilus zhejiangensis Z.Wei&Y.B.Chang, and prevents test-tube plantlets from generating excessive growth and vitrification phenomena, so as to obtain a large number of anoectochilus zhejiangensis Z.Wei&Y.B.Chang seedlings, and lay a foundation for scale production and application. The method includes an explant preparation stage, a primary induction culture stage, a secondary culture stage, a rooting culture stage and a transplanting stage; a primary induction culture medium and a secondary proliferation culture medium are each composed of the following components: MS, 0.1-0.5 mg / L of TDZ, 0.5-0.8 mg / L of 6-BA, 0.18-0.25 mg / L of NAA, 50-100 g / L of mashed potato, and 0.2-0.6 mg / L of activated carbon; a rooting culture medium is composed of the following components: MS, 0.6-1.5 mg / L of NAA, 50-100 g / L of mashed banana, and 0.4-1.0 mg / L of activated carbon.

Description

Technical field [0001] The invention relates to a method for propagating Zhejiang golden thread orchid seedlings, and belongs to the technical field of plant tissue culture rapid propagation. Background technique [0002] Zhejiang Golden Thread Orchid Anoectochilus zhejiangensis Z.Wei&Y.B.Chang, also known as Zhejiang Open-lip Orchid, is one of the rare and endangered orchid plants in my country and has been included in the list of national secondary protected plants and the list of rare and endangered plants in Zhejiang Province. Wild are distributed in Zhejiang (Suichang), Fujian (Jianyang, Jiangle), Guangxi (Longsheng) and other places. They are born in the dampness and humidity of mountain slopes or valleys at an altitude of 700-1200 meters. It is a traditional and precious herbal medicine in my country, and it is widely used in folk. [0003] Zhejiang golden thread orchid is a plant of the genus Orchidaceae. The micro-embryos of the seeds are immature and the seed germination...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈菁瑛刘保财
Owner 福建省农业科学院农业生物资源研究所
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