Unlock instant, AI-driven research and patent intelligence for your innovation.

Rapid proliferation medium for Dendrobium officinale tissue culture seedlings

A proliferation medium and technology of Dendrobium candidum, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve problems such as high nitrogen content, yellowing of lower leaves, growth retardation, etc., and achieve transplanting survival rate High, increase the multiplication coefficient, and the effect of strong stress resistance of seedlings

Active Publication Date: 2017-03-08
江苏丰收大地种业发展有限公司
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The concentration of total inorganic salts in MS medium is too high and the nitrogen content is high, resulting in slight yellowing of the lower leaves of the stem buds, growth retardation, and poisoning symptoms in stem buds; the concentration of total inorganic salts in N6 is high, nitrate nitrogen and ammonium nitrogen The ratio is not suitable, the lower leaves are severely yellowed, and the growth is retarded
So MS and N6 are not suitable as the basic medium for the subculture of Dendrobium officinale

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: prepare the culture medium of the formula that following composition is formed:

[0017] Huabao No. 1 0.50g / L, Huabao No. 2 0.50g / L, peptone 1.00g / L, inositol 0.05g / L, tricalcium phosphate 0.10g / L, banana 70g / L, potato 30g / L, fast Dadu 0.55ml / L, Aiduoshou 0.05ml / L, naphthaleneacetic acid 0.05mg / L, activated carbon (AC) 1.00g / L, white sugar 10g / L; pH 5.6; and add agar 5g / L.

[0018] Inoculate the seedlings of Dendrobium candidum into the medium for cultivation, the light time is 12h / d, the light intensity is 1500-1800lux, and the temperature of the cultivation room is controlled at about 25 degrees Celsius. After 2 months of cultivation, record the growth of the Dendrobium candidum tissue culture seedlings .

Embodiment 2

[0019] Embodiment 2: prepare the culture medium of the formula that following composition forms:

[0020] Huabao No. 1 2.50g / L, Huabao No. 2 2.50g / L, peptone 4.00g / L, inositol 0.20g / L, tricalcium phosphate 0.35g / L, banana 145g / L, potato 80g / L, fast Dadu 1.50ml / L, Aiduoshou 0.25ml / L, naphthaleneacetic acid 0.25mg / L, activated carbon (AC) 2.00g / L, white sugar 30g / L; pH 5.8; and add agar 5g / L.

[0021] Inoculate the seedlings of Dendrobium candidum into the medium for cultivation, the light time is 12h / d, the light intensity is 1500-1800lux, and the temperature of the cultivation room is controlled at about 25 degrees Celsius. After 2 months of cultivation, record the growth of the Dendrobium candidum tissue culture seedlings .

Embodiment 3

[0022] Embodiment 3: prepare the culture medium of the formula that following composition is formed:

[0023] Huabao No. 1 1.00g / L, Huabao No. 2 1.00g / L, peptone 1.50g / L, inositol 0.075g / L, tricalcium phosphate 0.15g / L, banana 80g / L, potato 40g / L, fast Dadu 0.75ml / L, Aiduoshou 0.10ml / L, naphthaleneacetic acid 0.10mg / L, activated carbon (AC) 1.25g / L, white sugar 15g / L; pH 6.2; and add agar 5g / L.

[0024] Inoculate the seedlings of Dendrobium candidum into the medium for cultivation, the light time is 12h / d, the light intensity is 1500-1800lux, and the temperature of the cultivation room is controlled at about 25 degrees Celsius. After 2 months of cultivation, record the growth of the Dendrobium candidum tissue culture seedlings .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rapid proliferation culture medium of dendrobium candidum tissue culture seedlings. The rapid proliferation culture medium of the dendrobium candidum tissue culture seedlings consists of components with the following proportions: 0.50-2.50g / L Hyponex No. 1, 0.50-2.50g / L Hyponex No.2, 1.00-4.00g / L peptone, 0.05-0.20g / L inositol, 0.10-0.35g / L tricalcium phosphate, 70-145g / L bananas, 30-80g / L potatoes, 0.55-1.50ml / L Sudaduo (a plant mineral element), 0.05-0.25ml / L atonik, 0.05-0.25mg / L naphthylacetic acid, 1.00-2.00g / L activated carbon (AC) and 10-30g / L white granulated sugar. By adopting the formula of the rapid proliferation culture medium of the dendrobium candidum tissue culture seedlings disclosed by the invention, the culture cycle can be shortened, and proliferation coefficients can be increased; and moreover, high transplanting survival rate of the tissue culture seedlings and strong stress resistance of the seedlings can be achieved.

Description

technical field [0001] The invention relates to the technical field of tissue culture of dendrobium candidum, in particular to a rapid proliferation medium for tissue culture seedlings of dendrobium candidum. Background technique [0002] Dendrobium candidum, also known as black knot grass and Tiepilan, is a perennial epiphytic herb of Orchidaceae, and is a precious wild medicinal plant. Dendrobium has the effects of anti-tumor, anti-aging, enhancing human immunity and dilating blood vessels. The reproductive capacity of Dendrobium candidum seeds is low under natural conditions, and the propagation speed is slow only by ramets, coupled with long-term human excavation, the wild resources of Dendrobium candidum are increasingly depleted. In the 1980s, Dendrobium officinale was listed as a rare and endangered medicinal plant under national key protection. [0003] In order to meet the needs of the market, many domestic researchers have studied the tissue culture technology of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈明亮周志疆张丽丽张桢
Owner 江苏丰收大地种业发展有限公司