A method for extracting oat beta-glucan by fermentation
A technology of glucan and fermentation method, applied in the field of extracting oat beta-glucan, can solve the problems of high cost and low extraction rate, and achieve the effects of low cost, simple steps and high yield
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Embodiment 1
[0020] Example 1. Fermentation and extraction of oat β-glucan by Aspergillus niger
[0021] The specific implementation steps are as follows:
[0022] 1) After grinding the oat grains, pass through a 50-mesh sieve, take the oat bran on the sieve, add 82% ethanol with a solid-liquid mass-volume ratio of 1:5, reflux in a water bath at 85 °C for 2 h, centrifuge at 1000 r / min for 5 min to remove ethanol Keep the precipitate and dry it in a blast drying oven at 40 °C for 12 hours to obtain oat bran;
[0023] 2) Prepare 5×10 6 A / mL Aspergillus niger spore suspension, preserved at 4 °C;
[0024] 3) Weigh 5 g of oat bran and autoclave, add sterile water at a solid-liquid ratio of 1:10 (W / V), and inoculate the Aspergillus niger spore suspension for fermentation. Fermentation conditions: fermentation time 10.30 h, inoculation amount 12 mL, fermentation temperature 42 °C, rotation speed 180 r / min;
[0025] 4) Collect the fermentation products and extract them in a 52 °C water bath with 400 r / min...
Embodiment 2
[0031] Example 2: Effect of fermentation time on the extraction of oat β-glucan by Aspergillus niger fermentation
[0032] In order to compare the influence of fermentation time on the yield of oat β-glucan, the fermentation time of step 3) in Example 1 was changed to 8, 12, and 16 h, and the remaining steps were the same as in Example 1.
[0033] The yield of oat β-glucan under different fermentation time is shown in Table 1:
[0034] Table 1 The yield of oat β-glucan under different fermentation times
[0035]
Embodiment 3
[0036] Example 3. The effect of inoculation amount on the extraction of oat β-glucan by Aspergillus niger fermentation
[0037] In order to compare the influence of the inoculation amount on the yield of oat β-glucan, the inoculation amount in step 3) in Example 1 was changed to 5, 10, and 15 mL, and the remaining steps were the same as in Example 1.
[0038] The yield of oat β-glucan under different inoculation amounts is shown in Table 2:
[0039] Table 2 The yield of oat β-glucan under different inoculation amounts
[0040]
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