Preparation capable of inhibiting Newcastle disease virus and application thereof
A technology of Newcastle disease virus and its preparation, which is applied in the field of marine biology, can solve the problems of high incidence and achieve the effects of reducing toxic side effects and related biological pollution, increasing RNA expression, and good biological safety
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] EID 50 Determination of the method: take 30 SPF chicken embryos, divide them into 6 groups, and inject 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 Chicken Newcastle disease Lasota virus stock solution, after incubation at 37°C for 72 hours, allantoic fluid was collected and EID was determined 50 =10 -8.45 / 0.2ml, take 150EID 50 is the amount of virus injected.
[0029] Determination of drug safety limit: α-chitosan with molecular weights of 9926, 8373, 4513, 2761, 2622, 2391, 1968, and 1729Da was prepared into three gradients of 15g / l, 10g / l, and 5g / l with physiological saline, respectively. The solution was injected into the allantoic cavity of SPF chicken embryos respectively, and within 96 hours of observation, they survived completely and continued to be cultured until hatching. The appearance of the chicken embryos was healthy and complete, without disability.
[0030] Low-concentration direct inhibition test: α-chitosan with molecular weights of 9926, 8373, ...
Embodiment 2
[0035] High-concentration direct inhibition test: α-chitosan with molecular weights of 9926, 8373, 4513, 2761, 2622, 2391, 1968, and 1729 Da was prepared into a 10 g / L solution with saline, and then acetic acid was added to promote dissolution. The final concentration of acetic acid in the solution reached 1 wt%. After further dissolving, the α-chitosan with different molecular weights is filtered and sterilized by means of physiological saline solution, and then it is set aside. Dilute lasota chicken Newcastle disease virus to 10 -6 After (primary virus titer is 2 11 , lead to death within 96h), and then mix the virus dilution with the solution of different concentrations of the above substances in a ratio of 1:1 (v / v), and number them in descending order according to the molecular weight of α-chitosan 1-8 groups, spare.
[0036] Take 9-11 day-old SPF chicken embryos, use an egg canister to observe the survival of the chicken embryos and draw the air chamber to determine t...
Embodiment 3
[0038]High-concentration prevention experiment: α-chitosan with molecular weights of 9926, 8373, 4513, 2761, 2622, 2391, 1968, and 1729 Da was prepared into a 10 g / L solution with physiological saline, and then acetic acid was added to promote dissolution. The final concentration of acetic acid in the solution reached 1 wt%. After further dissolving, the α-chitosan with different molecular weights was filtered and sterilized in a solution prepared with physiological saline, and numbered in groups 1-8 for use.
[0039] Take 9-11 day-old SPF chicken embryos, use an egg canister to observe the survival of the chicken embryos and draw the air chamber to determine the position of the hole. Use iodophor to sterilize the shell of the air chamber of the chicken embryo, and then sterilize it with 75% alcohol, and inject 0.2 mL / piece of the solution of groups 1-8 after punching holes. At the same time, the blank group was injected with normal saline, the positive control was 10 g / L rib...
PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More