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Microscope with External Fluorescence Module

A microscope and fluorescence technology, applied in the microscope field, can solve the problems of large light loss, small field of view of the light source, insufficient light intensity, etc., and achieve the effect of low price, strong light, and large spot area

Active Publication Date: 2016-01-27
宁波胤瑞生物医学仪器有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of traditional microscopes such as large light loss, small light source field of view, and insufficient light intensity, and provide a lighting device with a large light source field of view, strong light, and uniform light intensity. The technical solution is as follows:

Method used

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  • Microscope with External Fluorescence Module
  • Microscope with External Fluorescence Module
  • Microscope with External Fluorescence Module

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] refer to figure 1 with figure 2 As shown, the fluorescent module 1 includes an excitation filter 11 , a dichroic filter 12 and an emission filter 13 . The fluorescence module is placed between the objective lens 3 and the sample 2, and is located directly below the objective lens 3. The excitation filter 11 is perpendicular to the emission filter 13 at 90 degrees, and the dichroic filter 12 bisects the excitation filter 11 and the emission filter. The light sheet 13 forms a right angle, and the dichroic sheet 12 forms an angle of 45 degrees with the optical axis of the microscope.

[0027] First, the light emitted by the external light source 5 enters the fluorescent module 1, and is filtered by the excitation filter 11 to obtain light with a specific band and short wavelength. On the dichroic film 12, at this time, the dichroic dichroic film 12 shows reflection characteristics, and the short-wavelength light is reflected onto the sample 2, and after being excited on...

Embodiment 2

[0031] refer to image 3 with Figure 4 As shown, first, the light emitted by the external light source 5 passes through the diaphragm 6 and the lens 7, enters the fluorescent module 1, and is filtered by the excitation filter 11 to obtain short-wavelength light, which is emitted to the dichroic dichroic At this time, the dichroic film exhibits reflective properties, reflecting the short-wavelength light to the objective lens 3, and irradiating it to the sample 2 through the objective lens 3, and the long-wavelength light is excited on the sample 2 and then passes through the objective lens 3 enters the fluorescence module 1, passes through the emission filter 13, and then enters the eyepiece 4 or the detection device.

[0032] The light source path of a traditional microscope is an external light source, a fluorescent module, an objective lens, a sample, an objective lens, a fluorescent module, and an eyepiece.

[0033] The area of ​​the light spot of a traditional microsco...

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Abstract

The invention provides a novel microscope with an external fluorescent module. A lighting device of the microscope comprises the fluorescent module consisting of an external light source and an optical filter; the fluorescent module is arranged between an object lens and a sample, and is arranged under the object lens; the path of the light source of the microscope comprises the external light source, the fluorescent module, the sample, the fluorescent module, the object lens and an eye lens. Compared with the conventional microscope, the novel microscope with the external fluorescent module is very less in light loss, can realize sample lighting which is large in light spot, high in light intensity, uniform and bright, and is particularly suitable for biological signal detection with large area and weak fluorescent signals.

Description

technical field [0001] The invention relates to a microscope, in particular to a novel microscope with an external fluorescent module. Background technique [0002] The fluorescence module of the current fluorescence microscope is all between the objective lens and the eyepiece, using a mercury lamp, a xenon lamp or a halogen lamp as a light source, and the excitation light reaches the color filter group through the Kohler illumination system (the color filter group consists of an excitation filter , dichroic dichroic film, and emission filter), the light source is passed through the excitation filter to obtain the required excitation spectrum, reflected by the dichroic dichroic film and shot onto the sample through the objective lens, after the sample dyed by the fluorescent dye is excited, The emission wavelength is greater than the spectrum of the excitation light. The emitted light passes through the objective lens, the dichroic filter, and the emission filter (the spec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/06
CPCG02B21/06
Inventor 刘勇俞育德刘文文刘元杰李钊蒋莉娟
Owner 宁波胤瑞生物医学仪器有限责任公司