Method and special culture medium for inducing seedless grape young embryos to generate somatic embryos
A seedless grape, somatic embryo technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of difficult somatic embryos, low plant regeneration efficiency, etc. well-developed effect
Active Publication Date: 2015-09-02
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology
[0005] The purpose of the present invention is to provide a special medium for inducing somatic embryos from immature embryos of seedless grapes, which solves the problems of difficulty in inducing somatic embryos of seedless grapes and low efficiency of regenerating plants in the prior art
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[0022] The present invention will be described in detail below in combination with specific embodiments.
[0023] The invention provides a special culture medium for inducing seedless grape immature embryos to produce somatic embryos, which is named TL medium, and its components and contents are as follows: calcium nitrate 250.0 mg / L, potassium nitrate 600.0 mg / L, potassium chloride 75.0mg / L, ammonium nitrate 300.0mg / L, magnesium sulfate 1200.0mg / L, potassium dihydrogen phosphate 300.0mg / L, manganese sulfate 3.0mg / L, potassium iodide 0.8mg / L, boric acid 0.5mg / L, zinc sulfate 0.5 mg / L, sodium selenite 0.25mg / L, cobalt chloride 0.025mg / L, copper sulfate 0.025mg / L, sodium molybdate 0.025mg / L, iron citrate 10.0mg / L, thiamine hydrochloride 0.25mg / L, pyridoxine hydrochloride 0.25mg / L, D-calcium pantothenate 0.25mg / L, niacin 0.25mg / L, asparagine 300mg / L, glycine 5.0mg / L, arginine 2.0mg / L, muscle Alcohol 50.0mg / L, hydrolyzed casein 500.0mg / L, L-cysteine 121.16mg / L, sucrose 30000mg...
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The invention discloses a special culture medium for inducing seedless grape young embryos to generate somatic embryos. The culture medium is characterized in that in the process of ovule cultivation and somatic embryo generation induction, the same basic formula is adopted and different supplementary elements are added in different cultivation stages to achieve the expected effect. The invention further discloses a method for inducing the seedless grape young embryos to generate the somatic embryos. The method comprises the following steps: 6 weeks after blooming of a grape tree, collecting young fruits in the field, disinfecting, sequentially conducting ovule endodermal cultivation, inducing cultivation on embryonic callus and differentiation cultivation on the somatic embryos to obtain a somatic embryo in-vitro regenerated plant, and then conducting hardening seedling and transplanting to obtain a robust grape plant. The somatic embryos subjected to induced differentiation is more in number, ordered in development, and robust in growth after germinating to be a seedling, and has high possibility of surviving when transplanted.
Description
technical field [0001] The invention belongs to the technical field of plant cultivation, and specifically relates to a special culture medium for inducing somatic embryos from immature embryos of seedless grapes. The invention also provides a method for inducing somatic embryos from immature embryos of non-nucleated grapes. Background technique [0002] Grape is one of the oldest fruit tree species in the world. It has been cultivated for more than 5,000 years. It is loved by people because of its strong adaptability, high yield, rich nutrition and various health functions. In my country, grape production has always been an important aspect of agriculture. An important part of production. In recent years, my country's grape industry has developed rapidly, and the requirements for table grape varieties are getting higher and higher, especially seedless grapes. favored by those. Compared with other plants, the research and application of grape transgenic technology is relative...
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Inventor 田莉莉牛良
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI