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Method for treating agricultural waste generated by mandarin orange cultivation through microbiological method

A technology of agricultural waste and microbial method, applied in the field of agricultural waste treatment, can solve problems such as environmental pollution, and achieve the effects of reducing triggering factors, reducing usage, and low cost of raw materials

Inactive Publication Date: 2015-09-02
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the means to prevent the above problems is mainly through incineration, but this method will not only cause environmental pollution, but also fail to realize the comprehensive recycling of this huge amount of biomass resources, so it cannot be regarded as an ideal treatment method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for treating agricultural waste produced by tangerine cultivation by a microbial method, comprising the following steps:

[0027] (1) Preparation of crop waste powder: the fallen flowers, fallen leaves and dead branches of tangerines produced by tangerine cultivation are crushed, mixed through a 200-mesh sieve, and set aside;

[0028] (2) Seed cultivation: the above-mentioned prepared agricultural waste powder was added to sugarcane molasses alcohol waste liquid and urea at 20 ° Bx, the mass ratio of agricultural crop waste powder, sugarcane molasses alcohol waste liquid, and urea was 10:85:5, mixed After homogenization, sterilize at 110°C for 35 minutes as the seed medium, and after cooling, inoculate the spore concentration of 10% according to the mass ratio of 3%. 5 spores / mL cellulase-producing strain spore suspension, at 25°C, shake the flask at a shaking speed of 150r / min, and ferment for 12h to prepare seed culture; the cellulase-producing strain is Tric...

Embodiment 2

[0033] A method for treating agricultural waste produced by tangerine cultivation by a microbial method, comprising the following steps:

[0034] (1) Preparation of crop waste powder: the fallen flowers, fallen leaves and dead branches of tangerines produced by tangerine cultivation are crushed, mixed through a 200-mesh sieve, and set aside;

[0035] (2) Seed culture: the above-mentioned prepared agricultural waste powder is added to beet alcohol waste liquid and urea at 50 ° Bx, the mass ratio of agricultural waste powder, alcohol waste liquid, and urea is 20:75:5, and after mixing, Sterilize at 121°C for 45 minutes as the seed medium, and after cooling, inoculate the spore concentration of 10% according to the mass ratio of 10%. 7 spores / mL cellulase-producing strain spore suspension, at 37°C, shake the flask at a shaking speed of 250r / min, and ferment for 24h to prepare seed culture; the cellulase-producing strain is Trichoderma reesei;

[0036](3) Semi-solid expanded cult...

Embodiment 3

[0040] A method for treating agricultural waste produced by tangerine cultivation by a microbial method, comprising the following steps:

[0041] (1) Preparation of crop waste powder: the fallen flowers, fallen leaves and dead branches of tangerines produced by tangerine cultivation are crushed, mixed through a 200-mesh sieve, and set aside;

[0042] (2) Seed culture: the above-mentioned prepared crop waste powder is added to sugarcane molasses alcohol waste liquid and urea at 30 ° Bx, the mass ratio of crop waste powder, alcohol waste liquid, and urea is 14:85:1, after mixing After being sterilized at 115°C for 40 minutes as the seed medium, after cooling, inoculate the spore concentration of 10 according to the inoculation amount of 5% by mass. 6 Spores / mL cellulase-producing strain spore suspension, at 30°C, shake the flask at a shaking speed of 200r / min, and ferment for 18h to prepare seed culture; the cellulase-producing strain is Trichoderma lignin;

[0043] (3) Semi-so...

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PUM

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Abstract

The invention discloses a method for treating an agricultural waste generated by mandarin orange cultivation through a microbiological method. The method comprises the following steps of (1) preparing crop waste powder; (2) seed culture; (3) semisolid enlarged culture; (4) solid-state fermentation, and the like. The method disclosed by the invention can provide a new way for the treatment and recycling of waste alcohol liquid processed in a sugar industry and a large quantity of agricultural wastes generated in a mandarin orange culture process; a microbial fertilizer obtained through treatment can be used for increasing the biomass of biological prevention and control trichoderma in the environment, improving the microbe ecology of a mandarin orange garden, improving the soil, inhibiting the mandarin orange pathogenic microorganism in the microbe ecology in a garden and decreasing the triggering factor of a mandarin orange microbe disease by being applied to a mandarin orange garden, thereby reducing the usage amount of a pesticide and enhancing the quality and safety of a mandarin orange fruit.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides and environmental protection, and in particular relates to a microbial method for treating agricultural waste produced by tangerine cultivation. Background technique [0002] Tangerine fruit contains a variety of nutrients, such as sugar, citric acid, vitamin C, protein, carotene, vitamin P and other vitamins. The vitamin C content is 6-20 times that of apples. Vitamin C has a beauty effect, citric acid has the effect of relieving fatigue; the inner thin skin contains dietary fiber and pectin, which can promote laxatives and lower cholesterol; hesperidin can strengthen the toughness of capillaries, lower blood pressure, and expand the coronary artery of the heart Arteries, so oranges are food for preventing arteriosclerosis of coronary heart disease. Studies have confirmed that eating oranges can reduce cholesterol deposited in arteries and blood vessels, and help reverse atheroscler...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00B09B3/00
Inventor 王战
Owner GUANGXI UNIV