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A throwable one-step electrodeposition immunobiosensor for detecting histamine and its preparation method

A biosensor, electrodeposition technology, applied in the field of biosensors, can solve the problems of only one antigen active site, difficult to directly detect histamine, small molecular weight of histamine, etc., to achieve easy electron transfer, high sensitivity and simple operation. Effect

Active Publication Date: 2017-12-26
深圳深镧科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular weight of histamine is extremely small (111 Da) and there is only one antigenic active site, so it is difficult to directly detect histamine using an electrochemical immunosensor

Method used

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  • A throwable one-step electrodeposition immunobiosensor for detecting histamine and its preparation method
  • A throwable one-step electrodeposition immunobiosensor for detecting histamine and its preparation method
  • A throwable one-step electrodeposition immunobiosensor for detecting histamine and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This embodiment provides a disposable one-step electrodeposition immunological biosensor for detecting histamine, using an electrode with a Prussian blue-chitosan-nano-gold composite film on the surface, and a histamine coating agent is fixed on the electrode.

[0045] Nano gold and Prussian blue (Fe 4 [Fe(CN) 6 ] 3 The molar concentration ratio of) is 1:1000, and the chitosan content is 0.05%.

[0046] A method for preparing the above-mentioned immune biosensor includes the following steps:

[0047] S1. Dissolve 0.05 g of chitosan in 100 mL of 1.0% acetic acid solution, stir at room temperature for 3 hours to completely dissolve the chitosan, and prepare a 0.05% chitosan solution. Add 0.0406 g FeCl 3 , 0.0822g K 3 [Fe(CN) 6 ], 0.7455g KCl and 1mL concentrated hydrochloric acid were added to 100mL of the above chitosan solution, and ultrasonically dispersed at room temperature until a stable dark green dispersion was obtained; then 1mL of the prepared gold nanogel was added to...

Embodiment 2

[0055] This embodiment provides a disposable one-step electrodeposition immunological biosensor for detecting histamine, using an electrode with a Prussian blue-chitosan-nano-gold composite film on the surface, and a histamine coating agent is fixed on the electrode.

[0056] Nano gold and Prussian blue (Fe 4 [Fe(CN) 6 ] 3 The molar concentration ratio of) is 1:2000, and the chitosan content is 0.05%.

[0057] A method for preparing the above-mentioned immune biosensor includes the following steps:

[0058] S1. Dissolve 0.05 g of chitosan in 100 mL of 1.0% acetic acid solution, stir at room temperature for 3 hours to completely dissolve the chitosan, and prepare a 0.05% chitosan solution. Add 0.0813 g FeCl 3 , 0.1644 g K 3 [Fe(CN) 6 ], 0.7455g KCl and 1 mL of concentrated hydrochloric acid were added to 100 mL of the above chitosan solution, and ultrasonically dispersed at room temperature until a stable dark green dispersion was obtained; then 1 mL of the prepared gold nanogel was a...

Embodiment 3

[0065] This embodiment provides a disposable one-step electrodeposition immunological biosensor for detecting histamine, using an electrode with a Prussian blue-chitosan-nano-gold composite film on the surface, and a histamine coating agent is fixed on the electrode.

[0066] Nano gold and Prussian blue (Fe 4 [Fe(CN) 6 ] 3 The molar concentration ratio of) is 1:400, and the chitosan content is 0.01%

[0067] A method for preparing the above-mentioned immune biosensor includes the following steps:

[0068] S1. Dissolve 0.01g of chitosan in 100 mL of 1.0% acetic acid solution, stir at room temperature for 2 hours to completely dissolve the chitosan, and prepare a 0.01% chitosan solution. 0.0163 gFeCl 3 , 0.0329 g K 3 [Fe(CN) 6 ], 0.7455 g of KCl and 1 mL of concentrated hydrochloric acid were added to 100 mL of the above chitosan solution, and ultrasonically dispersed at room temperature until a stable dark green dispersion was obtained; then 1 mL of the prepared gold nanogel was added...

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Abstract

The invention belongs to the technical field of biosensors, and specifically discloses a throwable one-step electrodeposition immunobiological sensor for detecting histamine and a preparation method thereof. The immunobiological sensor of the present invention adopts the electrode with Prussian blue-chitosan-nano-gold composite film on the surface, and the histamine-coated original is fixed on the electrode; wherein, the molar concentration ratio of nano-gold and Prussian blue in the composite film is 1 :2000~1:20, the mass volume content of chitosan is 0.01~0.05%. Moreover, the one-step electrode deposition method is used to obtain a composite film on the electrode surface. The deposition step is simpler than the traditional polymer modified electrode preparation step, and it is easier to expose the antigen binding site. The immune biosensor of the present invention combines the competitive immune reaction with the biosensor, and can obtain a series of performance characterizations such as the histamine detection working curve according to the needs, has the advantages of simple operation, high sensitivity, fast and convenient, and can be used for the detection of histamine in samples. On-site rapid detection.

Description

Technical field [0001] The invention belongs to the technical field of biosensors, and more specifically, relates to a disposable one-step electrodeposition immune biosensor for detecting histamine and a preparation method thereof. Background technique [0002] my country is rich in fish resources and is also a major producer and consumer of aquatic products. Aquatic products and their processing occupy an important position in the national economy and consumption. Histamine is formed by the decarboxylation reaction of free histidine under the catalysis of histidine decarboxylase. It is a toxic biological amine that is widely present in various organisms and foods, mainly aquatic products. Especially the pelagic sea fish with the characteristics of green skin and red meat and its processed products. Studies have shown that excessive intake of histamine can cause a series of allergic reactions such as headache, nausea, nausea and vomiting, diarrhea, heart palpitations, pruritic r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N33/53
Inventor 徐振林孙远明董秀秀梁祖培沈玉栋杨金易王弘雷红涛
Owner 深圳深镧科技有限公司