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Liquid biocontrol agent for wheat scab and preparation method thereof

A technology for wheat scab and biocontrol fungicides, applied in biochemical equipment and methods, botanical equipment and methods, microorganism-based methods, etc., can solve problems such as loss and production failure, and achieve good control effects

Inactive Publication Date: 2018-03-06
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In a pandemic year, the production is generally reduced by 10-20%, and in severe cases it reaches 80-90%, or even goes out of production, causing huge losses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Isolation and screening of bacterial strain LM3403

[0016] Collect wild yak dung that grows in the alpine and pollution-free grassland above 3,000 meters above sea level. The method of gradient dilution and coating separation is used to separate the bacterial strains in the sample by coating. Colonies with obvious morphological differences were picked for streak purification, numbering, and preservation for future use. The antibacterial ability of the tested strains was investigated by filter paper method and plate confrontation method, and the strain with the strongest broad-spectrum antibacterial ability was named LM3403. The width of the inhibition zone of strain LM3403 against Fusarium graminearum was (6.7±1.1) mm.

Embodiment 2

[0017] Embodiment 2: identification of bacterial strain LM3403

[0018] The colony of strain LM3403 on the NA plate is round, opaque and milky white, sticky, and the edges are irregular; the cells are rod-shaped, Gram-positive, spore-shaped, and oval. Aerobic growth: positive, motility: positive, V-P test: positive, catalase test: positive, indole test: positive, starch hydrolysis: positive, casein hydrolysis: positive, gelatin liquefaction: positive, glucose fermentation test: Positive, nitrate reduction: positive, urea utilization: positive, the morphological characteristics, physiological and biochemical characteristics of the strain LM3403 are consistent with the standard strain of Bacillus subtilis. The 16S rDNA sequence of strain LM3403 and Bacillus subtilis strain SPA N1(JQ308575), Bacillus subtilis strain ME2(JQ900623) and Bacillus subtilis The homology of strain BDT3(HQ711982) reached 100%. Comprehensive analysis determined that the strain LM3403 was Bacill...

Embodiment 3

[0019] Example 3: Extraction and analysis of bacterial strain LM3403 antibacterial lipopeptide

[0020] Centrifuge the fermentation broth of the strain at 4°C and 12000rpm for 15min, collect the sterile supernatant; use 6mol / L HCl to adjust the sterile supernatant to pH2.0, let stand overnight at 4°C, and centrifuge at 12000r / min at 4°C After 15 minutes, collect the precipitate; add an appropriate amount of methanol to the precipitate, stir on a magnetic stirrer for 30 minutes, put it in a refrigerator at 4°C for extraction for 8 hours, and centrifuge at 12,000 rpm for 15 minutes at 4°C after the extraction is complete to obtain a methanol extract. After concentration under reduced pressure and vacuum freeze-drying, the antibacterial substance was obtained and stored at -20°C. The antibacterial substance after vacuum freeze-drying was taken, dissolved in phosphate buffer solution with pH 7.0, prepared into a certain concentration antibacterial substance solution, and stored at...

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Abstract

The invention belongs to the fields of microorganisms and agricultural disease prevention and control, and discloses a liquid biocontrol agent for wheat scab and a preparation method thereof. The effective active ingredient of the biocontrol agent is composed of Bacillus subtilis and its fermentation metabolites, biochemical fulvic acid and chitosan oligosaccharide, etc., and the Bacillus subtilis is preferably preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee Bacillus subtilis LM3403 with the preservation number of CGMCC No. 9377. The effective components of the biocontrol fungicide synergistically prevent and control wheat scab through different modes of action, have better control effect, and are environmentally friendly, have no residue, no pollution and no drug resistance.

Description

technical field [0001] The invention belongs to the fields of microorganisms and agricultural disease prevention and control, and in particular relates to a liquid biocontrol agent for wheat scab and a preparation method thereof. Background technique [0002] Wheat head blight is a fungal disease caused by Fusarium graminearum infection. It has a strong pathogenicity and can infect all parts of wheat, mainly damaging the ears; it can occur at all stages of wheat growth, and infection at seedling stage Cause seedling rot, middle and late infection causes stalk rot and ear rot, especially ear rot is the most harmful. In a pandemic year, the production is generally reduced by 10-20%, and in severe cases it reaches 80-90%, or even goes out of production, causing huge losses. Wheat head blight not only causes a decrease in wheat yield, quality, and flour yield, but the metabolites of the bacteria contain mycotoxins such as deoxynivalenol and zearalenone, which can cause human an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A61P3/00C12N1/20C12R1/125
Inventor 陈亮关二旗李瑞静王金水卞科
Owner HENAN UNIVERSITY OF TECHNOLOGY