Enterococcus mundtii and application thereof in low-temperature ensiling
A technology of Enterococcus monsonii and silage, applied in the field of microorganisms, can solve problems such as difficulty in fermenting high-quality feed and unfavorable growth of lactic acid bacteria
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Embodiment 1
[0040] Example 1, Isolation and identification of Enterococcus mundtii (Enterococcus mundtii) QH1-255
[0041] 1. Isolation and screening of strain QH1-255
[0042] Take 10g of wild wheat from Jianzha County, Qinghai Province, China, put it into 90mL of sterile water, shake for 10 seconds, draw 1mL of liquid into a 1.5mL centrifuge tube, and dilute 10 1 , 10 3 , 10 5 Take 20 μL of the diluted liquid and spread it on the MRS solid medium, culture anaerobically at 30°C for 48 hours, take a single colony MRS solid medium for expansion culture, and one of the obtained strains is numbered QH1-255.
[0043] Wherein, the solvent of the MRS solid medium is water, the solute and its concentration are as follows: peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, glucose 20g / L, sodium acetate trihydrate 5g / L, spit Temperature 801g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 2g / L, manganese sulfate 0.25g / L, agar 17g / L.
[0044] 2. Identificati...
Embodiment 2
[0057] Embodiment 2, the growth of Enterococcus mundtii (Enterococcus mundtii) QH1-255 under different temperature, pH and salt concentration
[0058] 1. Growth of Enterococcus mundtii QH1-255 at different temperatures
[0059] Inoculate activated Enterococcus mundtii QH1-255CCTCC NO: M 2015253 in MRS liquid medium, culture at 5, 10, 45 and 50°C for 24 hours, and measure the light absorption at 600nm with a spectrophotometer value (OD600) to observe the growth of Enterococcus mundtii QH1-255 at different temperatures.
[0060] Wherein, the solvent of MRS liquid medium is water, solute and its concentration are as follows: peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, glucose 20g / L, sodium acetate trihydrate 5g / L, Tween 801g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 2g / L, manganese sulfate 0.25g / L; pH6.8.
[0061] The results are shown in Table 2. It can be seen that Enterococcus mundtii QH1-255CCTCC NO: M 2015253 grows well at...
Embodiment 3
[0072] Embodiment 3, the antibacterial activity assay of Enterococcus monsonii (Enterococcus mundtii) QH1-255
[0073] Bacteria tested: Micrococcus luteus, Micrococcus luteus, Salmonella and Escherichia coli.
[0074] 1. Inoculate the activated Enterococcus mundtii QH1-255CCTCC NO: M 2015253 in MRS liquid medium (same formula as above, pH 6.8), culture at 30°C for 48 hours, centrifuge the fermentation broth at 10,000rpm for 5min, take Serum was used for measurement.
[0075] 2. Measure its antibacterial activity by the Oxford cup double-layer plate method, heat the sterilized agar medium to completely melt, pour it into a petri dish, 15ml per dish (lower layer), and wait for it to solidify. In addition, cool the melted PDA medium to about 50°C and mix it with the bacteria to be tested, and add 5 ml of the culture medium mixed with the bacteria to the solidified medium to be solidified (upper layer). Place an Oxford cup (a round tube with an inner diameter of 6 mm, an outer d...
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