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Efficient continuous frequency mixing ultrasonic cell disruption treatment device

A treatment device and cell technology, applied in sludge treatment, water/sludge/sewage treatment, mechanical oscillation water/sewage treatment, etc., can solve the problem that the efficiency of sample treatment cannot meet the requirements of industrialization, the selection of ultrasonic transducers is single, Breaking the wall can not be realized at the same time, to achieve high engineering application value, wide application value, and improve the effect of ultrasonic cavitation efficiency

Active Publication Date: 2015-09-30
哈尔滨优脉雷声科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, various ultrasonic treatment devices for breaking biological cells have appeared one after another, but the following problems generally exist: the choice of ultrasonic transducer is single, the ultrasonic power and frequency are fixed, and the breaking of different biological cells cannot be achieved at the same time; The combination of ultrasound and other chemical methods improves the efficiency of wall breaking, but causes secondary pollution and other problems; the power of ultrasound is low, and the rate of wall breaking is low. Although it can effectively inhibit the growth of biological cells, it cannot fundamentally destroy cells. , resulting in sample processing efficiency not meeting industrial requirements

Method used

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  • Efficient continuous frequency mixing ultrasonic cell disruption treatment device

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Experimental program
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Effect test

Embodiment 1

[0021] Take 200ml of Microcystis aeruginosa solution with the same concentration in four groups A, B, C, and D, and the ultrasonic output power is 500W. Groups A, B, and C use ultrasonic waves with frequencies of 20KHz, 28KHz, and 35KHz respectively. For 15 seconds, group D uses 20kHz and 28kHz frequency mixing, 28kHz and 35kHz frequency mixing, and 20kHz and 35kHz frequency mixing for 5 seconds respectively, puts the algal liquid subjected to ultrasonication in the incubator, and takes the supernatant to measure the absorbance (OD value), compared with the control group without ultrasonic action, thereby quantitatively obtaining the biological content of cyanobacteria in the solution, as shown in Table 1.

[0022] Table 1

[0023]

[0024] It can be seen from Table 1 that the use of mixed-frequency ultrasound to treat cyanobacteria can effectively reduce the biological content of cyanobacteria in the solution, thereby increasing the removal rate, and the removal e...

Embodiment 2

[0026] Take 200ml of each Microcystis aeruginosa solution with the same concentration in groups A, B, C, and D, and adjust the ultrasonic output power to 500W. For 30 seconds, place the algae solution subjected to ultrasonication in the incubator, take the supernatant to measure the absorbance (OD value), and compare it with the control group without ultrasonication, so as to quantitatively obtain the biological content of cyanobacteria in the solution, such as Table 2 shows.

[0027] Table 2

[0028]

[0029] As can be seen from Table 2, the blue-green algae are treated with frequency-mixing ultrasound. With the increase of the action time of frequency-mixing ultrasound, the biological content of blue-green algae in the solution decreases, and the corresponding removal rate increases. Since the method is designed as a continuous treatment method, The ultrasonic action time will not be increased indefinitely, so a reasonable time range can be selected according to n...

Embodiment 3

[0031] Take 200ml of each Microcystis aeruginosa solution with the same concentration in four groups A, B, C and D, and the ultrasonic output power is 0.5kW, 1kW, 2kW, 3kW respectively. Put it still in the incubator, take the supernatant to measure the absorbance (OD value), and compare it with the control group without ultrasound, so as to quantitatively obtain the biological content of cyanobacteria in the solution, as shown in Table 3.

[0032] table 3

[0033]

[0034] It can be seen from Table 3 that the cyanobacteria were treated with frequency-mixing ultrasound. With the increase of the power of frequency-mixing ultrasound, the biological content of cyanobacteria in the solution was greatly reduced, and the corresponding removal rate increased.

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Abstract

The invention relates to an efficient continuous frequency mixing ultrasonic cell disruption treatment device which comprises a cell disruption ultrasonic reaction cavity. A feed port and a discharging port are formed in the two ends of the reaction cavity. The ultrasonic reaction cavity comprises multiple cylinder reaction cavities connected through pipelines, and at least two high-power ultrasonic transducers of different frequencies are arranged in each cylinder reaction cavity. Samples to be processed are diluted into a liquid state and pumped in through a feed port of a reaction container and a flow control valve, and the samples flow around in the reaction cavities. The high-power ultrasonic transducers arranged face to face are embedded into the two sides of each reaction cavity, the two ultrasonic transducers of each set act face to face to achieve ultrasonic wave frequency mixing, synthetic ultrasonic waves can achieve different frequencies and sound intensities, and the purpose of continuous and efficient cell disruption is achieved. The ultrasonic cell disruption treatment device is easy to operate, easy to achieve, high in efficiency and strong in continuity, and has high application value for cell disruption treatment of algae and other creatures.

Description

technical field [0001] The invention relates to the technical field of ultrasonic treatment, in particular to an efficient continuous frequency mixing ultrasonic cell wall breaking treatment device. Background technique [0002] With the development of social economy, environmental issues have been put on the agenda of governments at all levels as an unavoidable and important issue. Protecting the environment, reducing environmental pollution, and curbing the trend of ecological deterioration have become important tasks for the government's social management. In many pollution control studies, such as the flooding of algae plants in water bodies, it is necessary to destroy the plant cell walls of algae, so that the intracellular substances can flow out, so as to achieve the purpose of controlling algae pollution; in some sludge treatment processes, due to the presence of large amounts of Biological cells, the water combined in various forms makes the water content of the sl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/36C02F11/00
Inventor 杨浩
Owner 哈尔滨优脉雷声科技有限责任公司