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Energy-saving low-temperature heparin sodium extraction technology

A heparin sodium and process technology, which is applied in the field of biomedicine, can solve the problems of short holding time, incomplete enzymatic hydrolysis, and large coal-burning pollution, and achieves the effects of not easy inactivation, strong enzyme activity, and energy saving.

Active Publication Date: 2015-09-30
DAYING COUNTY TIANFENG BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the traditional production process, it is necessary to first heat up to 50-55 degrees, keep warm for 2 hours, then add 3-4% sodium chloride, and heat up to 85-95 degrees, in order to solve the problem of incomplete enzymolysis due to short holding time To overcome, 520kg-580kg of coal is required to extract 1kg of fuel, so a lot of energy is consumed, and coal burning causes more pollution to the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Pour clean water into the small intestine of the pig, and use a casing machine to squeeze out the small intestinal mucosa to the mucosa pool; wind the casing to form a casing handle, then put the casing casing into the soaking liquid for the first time soaking for 4 hours, take it out and drain it, and use the casing Special salt is used to marinate the casings, and then put the casings into the soaking solution for a second soak for 8 hours. The soaking solution for the first soaking is a saturated sodium chloride solution, and the soaking solution for the second soaking is a salinity of 15° Sodium chloride solution; when soaking for the first time and for the second time, soak 155 casings per 1000L of soaking liquid, collect the leachate for the first time for use, and collect the leachate for the second time as the casing for the next extraction Soaking liquid for the first soak; add the pig small intestinal mucosal slurry to the enzymatic hydrolysis tank, the pig sma...

Embodiment 2

[0023] Pour clean water into the small intestine of the pig, and use a casing machine to squeeze out the small intestinal mucosa to the mucosa pool; wind the casing to form a casing handle, then put the casing into the soaking liquid for the first time soaking for 4.5 hours, take it out and drain it, and use the casing Special salt is used to marinate the casings, and then put the casings into the soaking solution for a second soak for 7 hours. The soaking solution for the first soaking is a saturated sodium chloride solution, and the soaking solution for the second soaking is a salinity of 16° Sodium chloride solution; for the first soaking and second soaking, soak 160 casings in every 1000L soaking solution, collect the leachate from the first soaking for use, and collect the leachate after the second soaking as the casing for the next extraction Put the soaking solution soaked for the first time; add the pig small intestinal mucosal slurry into the enzymolysis tank, the pig ...

Embodiment 3

[0025]Pour clean water into the small intestine of the pig, and use a casing machine to squeeze out the small intestinal mucosa to the mucosa pool; wind the casing to form a casing handle, and then put the casing casing into the soaking liquid for the first time soaking for 5 hours, take it out and drain it, and use the casing Special salt is used to marinate the casings, and then put the casings into the soaking solution for a second soak for 6 hours. The soaking solution for the first soaking is a saturated sodium chloride solution, and the soaking solution for the second soaking is a salinity of 18° Sodium chloride solution; when soaking for the first time and for the second time, soak 165 pieces of casings per 1000L of soaking liquid, collect the leachate for the first time for use, and collect the leachate for the second time as the casing for the next extraction Soaking liquid for the first soak; add the pig small intestinal mucosal slurry to the enzymolysis tank, the pig...

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PUM

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Abstract

The invention provides an energy-saving low-temperature heparin sodium extraction technology. The technology includes the following steps that intestinal mucosae are prepared, casings are soaked in water, enzymolysis is performed, adsorption is performed, elution is performed, precipitation is performed, dewatering and drying are performed, 2709 protease is added in the small intestinal mucosae of pigs, the temperature is raised to 50-55 DEG C, heat preservation is performed for 8-12 h, 3-4% sodium chloride is added in, the temperature is raised to 60-65 DEG C, heat preservation is performed for 1 h, and filtering is performed; adsorption is performed for 8 h in filter liquor and strong basic ion exchange resin, resin is filtered out and cleaned up, a 20% sodium chloride solution is added in for performing elution two times, eluant is precipitated for 12 h through ethanol, the ethanol is filtered to be dry, and the heparin sodium is obtained. Compared with the prior art, about 200 kg of fuel can be saved when 1 kg of heparin sodium crude product is extracted, and fuel cost is reduced by 30-40%.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an energy-saving and low-temperature extraction process of heparin sodium. Background technique [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, mainly exists in mast cells, has anticoagulant effect, and is widely used in the treatment of surgery, cerebral thrombosis, myocardial infarction, etc. The molecular weight of heparin is about 3000-37500, and research on commercial heparin suggests that there are at least 21 molecular entities. Heparin sodium is the sodium salt of heparin. As a natural anticoagulant substance, heparin sodium has been valued by countries all over the world. It is also one of the main export drugs in my country. With the deepening of research, people found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid regulation functions, but also has anti-inflammatory, anti-allergic, anti-viral, anti-cancer a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 邓军肖莉
Owner DAYING COUNTY TIANFENG BIOLOGICAL PROD