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Schizochytrium limacinum fermentation broth wall-broken cell wall flocculate recovery method

A technology of Schizochytrium and recovery method, which is applied in the direction of fat production, microbial dissolution, fat oil/fat production, etc., to achieve the effect of increasing yield and obvious economic benefits

Inactive Publication Date: 2015-10-28
XIAMEN HUISON BIOTECH
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Problems solved by technology

The use of related wall-breaking technology and Schizochytrium powder in aquaculture has also gradually realized industrial production and promotion. , 200810047859.2, 200910033869.5, 200910111657.4, 200910225296.6, 201110077030.9, 201210491610.7, 200910226049.8, etc.) Involving microalgae and schizoamide fermentation liquid, the traditional method of flocculation (inorganic flocculant, poly There is no reported precedent for the recovery process of the broken cell wall (including protein, colloid, etc.) of Schizochytrium by flocculation of polysaccharide acid solution

Method used

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  • Schizochytrium limacinum fermentation broth wall-broken cell wall flocculate recovery method

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Embodiment Construction

[0015] Such as figure 1 As shown, the present invention discloses a flocculation recovery method of broken cell wall of Schizochytrium fermentation broth.

[0016] Firstly, the DHA-rich Schizochytrium fermented liquid is put into the broken wall tank, and the compound enzyme enzymolysis is carried out to break the wall and inactivate. The single cell of Schizochytrium is broken, and the DHA oil is released. At this time, the cell wall keeps more than 85% Broken rate.

[0017] Enzymolysis method: control the temperature at 35-60°C, add 1.0-4.0% compound enzyme by weight of the fermentation broth of Schizochytrium, heat and stir for 6.0-8.0 hours and inactivate the enzymatic hydrolysis, the inactivation temperature is 65-95°C; the compound enzyme It is a mixture of cellulase and neutral protease, the mixing ratio is (5-50%): (95-50%).

[0018] Then, the wall-breaking solution is flocculated and separated by a centrifuge, and the oil phase obtained is the primary oil rich in DH...

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Abstract

The invention discloses a schizochytrium limacinum fermentation broth wall-broken cell wall flocculate recovery method. The method comprises the steps that firstly, schizochytrium limacinum fermentation broth rich in DHA is fed into a wall breaking tank and is subjected to enzymolysis wall breaking through compound enzymes and inactivation, schizochytrium limacinum single cells are broken, and DHA oil is released; secondly, the PH of wall breaking liquid is controlled to be 6.0-6.5, a flocculating agent acid solution accounting for 2-6% of the self weight of the wall breaking liquid is added into the wall breaking liquid, and the mixture is stirred and is subjected to still standing and flocculation; finally, the mixture is subjected to separation through a centrifugal machine, an obtained oil phase is DHA-enriched primary grease, and an obtained slag phase is wall-broken cell wall flocculate. Through the method, the yield of the DHA-enriched primary grease can be effectively increased by 3-5%, the overall yield can reach 93-97%, the economic benefits are obvious, and the practicality and the environmental protection effect are achieved.

Description

technical field [0001] The invention relates to a recovery method for flocculating and recovering the broken cell wall from the broken wall of the Schizochytrium fermented liquid. technical background [0002] Schizochytrium, also known as Schizochytrium, belongs to a class of marine fungi belonging to Eumycota, Oomycetes, Saprolegniales, and Thraustochytriaceae. It is single-celled and spherical. Schizovibrio cells can accumulate a large amount of active substances useful to the human body, such as: the DHA content in its total lipids is very high, reaching 35% to 68%, and more than 90% of the fatty acids are neutral in the form of triglycerides. fat. Fatty acids with similar structures have low content and are easy to separate and purify. And its cell wall is rich in many beneficial substances such as protein and immune polysaccharide. Many experts and scholars in animal husbandry and aquaculture are also conducting research on the biological effects of DHA. The cell wa...

Claims

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Application Information

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IPC IPC(8): C11B1/00C12N1/06
Inventor 陈礼毅林杰陈水荣
Owner XIAMEN HUISON BIOTECH
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