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Micromolecule walnut peptide and preparation method thereof

A technology of walnut peptides and small molecules, applied in the direction of peptides, fermentation, etc., can solve the problems of waste discharge polluting the environment, low added value, etc., and achieve the effect of high content

Inactive Publication Date: 2015-10-28
BEIJING TIANTAI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are dozens of walnut oil manufacturers in my country, and a large amount of defatted walnut residue is used as feed and fertilizer or discarded, and the waste discharge pollutes the environment, and its added value is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of preparation method of small molecule walnut peptide of the present embodiment, comprises the following steps:

[0027] 1) Protein extraction: The process of protein extraction is to degrease walnuts to obtain degreased walnut dregs, crush the defatted walnut dregs to 40 mesh, add deionized water 5 times the weight of walnut dregs, add sodium bicarbonate solution or hydroxide Sodium solution adjusts the pH to 9, soaks for 2 hours, removes the lower precipitate with a centrifuge; adds citric acid solution or glacial acetic acid solution to adjust the pH of the centrifuged liquid to 4.2, centrifuges after standing for 3 hours, removes the lower precipitate and washes until neutral, The walnut protein extract is obtained, and the amount of walnut protein in the walnut protein extract is detected by Baume meter or Kjeldahl method. This example makes full use of the by-product of peach kernel oil extraction—defatted walnut dregs to prepare small molecular peptides, ...

Embodiment 2

[0038] A kind of preparation method of small molecule walnut peptide of the present embodiment, comprises the following steps:

[0039] 1) Protein extraction: The process of protein extraction is to degrease walnuts to obtain degreased walnut dregs, crush the defatted walnut dregs to 40 mesh, add deionized water 8 times the quality of walnut dregs, add sodium bicarbonate solution or hydroxide Sodium solution adjusts the pH to 8.5, soaks for 1 hour, and removes the lower precipitate with a centrifuge; adds citric acid solution or glacial acetic acid solution to adjust the pH of the centrifuged liquid to 4, centrifuges after standing for 1 hour, removes the lower precipitate and washes it to neutrality, The walnut protein extract is obtained, and the amount of walnut protein in the walnut protein extract is detected by Baume meter or Kjeldahl method. This example makes full use of the by-product of peach kernel oil extraction—defatted walnut dregs to prepare small molecular pept...

Embodiment 3

[0050] A kind of preparation method of small molecule walnut peptide of the present embodiment, comprises the following steps:

[0051] 1) Protein extraction: The process of protein extraction is to degrease walnuts to obtain defatted walnut residue, crush the defatted walnut residue to 60 mesh, add deionized water 8 times the weight of walnut residue, add sodium bicarbonate solution or hydroxide Sodium solution adjusts the pH to 9, soaks for 1 hour, removes the lower precipitate with a centrifuge; adds citric acid solution or glacial acetic acid solution to adjust the pH of the centrifuged liquid to 4.5, centrifuges after standing for 3 hours, removes the lower precipitate and washes until neutral, The walnut protein extract is obtained, and the amount of walnut protein in the walnut protein extract is detected by Baume meter or Kjeldahl method. This example makes full use of the by-product of peach kernel oil extraction—defatted walnut dregs to prepare small molecular peptid...

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Abstract

The invention relates to a micromolecule walnut peptide and a preparation method thereof. The preparation method sequentially comprises the following steps of protein extraction, twice enzymolysis, filtering, purification, concentration and drying. In the twice enzymolysis process, alkaline protease with the mass being 1-3% that of the walnut protein is added into a walnut protein extracting solution for once enzymolysis; enzyme deactivation is performed on a once enzymolysis solution after the once enzymolysis is finished; one or more kinds of proteinase including the flavored proteinase, the neutral proteinase and the papain with the mass being 1-3% that of the walnut protein are added after the once enzymolysis solution obtained after enzyme deactivation is performed is cooled for secondary enzymolysis. According to the preparation method, the twice enzymolysis method is adopted, due to the specificity of the structure of the walnut protein structure, sufficient enzymolysis cannot be performed on the walnut protein through enzymolysis of one proteinase, and the twice enzymolysis method is adopted for ensuring that the enzymolysis is brought into play on the most suitable condition of each proteinase, and therefore the obtained walnut peptide content is high.

Description

technical field [0001] The invention relates to the field of bioactive substance extraction, in particular to a small molecule walnut peptide and a preparation method thereof. Background technique [0002] Walnut is also called walnut, Qiang peach, long live seed, etc., and is a plant of the genus Juglandaceae. The oil content in the hickory nut can reach 60%-70%, wherein the unsaturated fatty acid content is more than 90%, the protein content of the hickory nut is 14%-17%, and the digestibility reaches 87.2%. But ideal hickory nut health food is rare on the market at present, except that it is made into canned food, moon cake stuffing, walnut powder, walnut milk, walnut oil, the development of other purposes is still blank, especially the deep processing product is less . Walnuts are more and more widely used in daily life and industrial production, but the problems they face are becoming more and more obvious. At present, there are dozens of walnut oil manufacturers in ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/30C07K2/00
Inventor 李俊伟
Owner BEIJING TIANTAI BIOTECH
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