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A kind of mercury-high-density lipoprotein chelate and its preparation method and application

A high-density lipoprotein and chelate technology, applied in the field of immunological detection of heavy metal ions, can solve problems such as mercury pollution, and achieve good repeatability and high accuracy

Inactive Publication Date: 2018-01-05
广州锦海骢健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] For the serious problem of mercury pollution, the object of the present invention is to provide a kind of mercury-high-density lipoprotein chelate and preparation method thereof, and establish the qualitative and quantitative detection method of mercury-high-density lipoprotein chelate, so that quantitative detection The Application of Mercury-High Density Lipoprotein Chelate in Evaluating the Degree of Mercury Pollution in a Region

Method used

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  • A kind of mercury-high-density lipoprotein chelate and its preparation method and application
  • A kind of mercury-high-density lipoprotein chelate and its preparation method and application
  • A kind of mercury-high-density lipoprotein chelate and its preparation method and application

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preparation example Construction

[0058] The present invention also provides the preparation method of mercury-high-density lipoprotein chelate, comprising the following steps:

[0059] A) Synthesis of mercury-high-density lipoprotein: adding mercury ions to the high-density lipoprotein purified from the human body or reorganized according to biological methods for chelation reaction to obtain a reaction solution;

[0060] B) Purification of mercury-high-density lipoprotein chelate: use immunoaffinity chromatography to remove unreacted high-density lipoprotein, specific antibodies and mercury ions in the reaction solution in step A), to obtain mercury-high-density lipoprotein Protein chelate, specifically comprises the following steps:

[0061] (1) dissolving sample: the mercury-high-density lipoprotein chelate of above-mentioned step A) is dissolved in normal saline, obtains the solution of mercury-high-density lipoprotein chelate;

[0062] (2) Equilibrium chromatography column: Use the dilution buffer to fl...

Embodiment 1

[0145] Example 1: Synthetic synthesis of mercury-high-density lipoprotein chelate comprises the following steps:

[0146] The mercury-high-density lipoprotein chelate prepared in this example was further separated by gel electrophoresis, and qualitatively identified by inductively coupled plasma mass spectrometry or atomic absorption spectrometry.

[0147] The method for preparing mercury-high-density lipoprotein chelate comprises the following steps:

[0148] 1) Borate buffer solution, its molar concentration is 0.01M, its preparation method is illustrated as follows: Weigh 0.31g of boric acid and dissolve it in 400mLddH 2 In O, the pH was adjusted to 9.0 with 0.1M NaOH, and the volume was adjusted to 500 mL.

[0149] 2) EDTA-NaHCO 3 Solution, its preparation method is as follows: Take 1.86g EDTA·2H 2 O and 16.8 g NaHCO 3 , dissolved in 900mLddH 2 In O, adjust the pH to 8.0 with 1.0M NaOH, dilute to 1000mL, autoclave, and store at room temperature;

[0150] 3) ITCBE w...

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Abstract

The invention provides a mercury-high-density lipoprotein chelate and its preparation method and application. It is chelated and can be used to prepare reagents for detecting human mercury-high-density lipoprotein chelate. The present invention proves for the first time that mercury ions can directly act on high-density lipoprotein. The invention establishes a qualitative and quantitative detection method for mercury-high-density lipoprotein chelate to detect the content of high-density lipoprotein in a population in a region, thereby indirectly reflecting the degree of mercury pollution in a region and the health impact on the population. The mercury-high-density lipoprotein chelate quantitative detection method established by the invention has high accuracy and good reproducibility.

Description

technical field [0001] The invention relates to immunological detection of heavy metal ions, in particular to a mercury-high-density lipoprotein chelate and its preparation method and application. Background technique [0002] Lipoprotein (lipoproteins) is a lipid-protein complex formed by the combination of protein and lipid, which can be divided into high density lipoprotein (high density lipoprotein, referred to as HDL), intermediate density lipoprotein (referred to as IDL), low density lipoprotein (low density lipoprotein, referred to as LDL), very low density lipoprotein (very low density lipoprotein, referred to as VLDL), chylogranules (chylomicron, CM). About 30% of the cholesterol in the blood is transported from tissue cells to the liver by high-density lipoprotein and converted into bile acids or excreted through bile. HDL consists of 50% protein, 30% phospholipids, 25% cholesterol (70% of which is cholesteryl ester), and 5% triacylglycerol. [0003] High-density...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N33/53G01N27/62G01N1/28G01N1/34G01N27/447G01N27/626
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 广州锦海骢健康科技有限公司
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