Method for obtaining heat-resisting morning glory plant
A morning glory and plant technology, which is applied in the field of obtaining heat-resistant mutant plants of morning glory, can solve the problems of poor heat and humidity resistance, easy disease and wither, achieve strong heat resistance, widen time and geographical scope, and shorten breeding time. Effect
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Embodiment 1
[0016] The method for obtaining morning glory mutant plants, the steps are as follows:
[0017] (1) Morning glory seedlings are sown and raised in early June. Nutrient pots or flowerpots can be used to raise seedlings. Select 8-10 leaves, grow vigorously and vigorously, and 600 seedlings without diseases and insect pests, and remove the top parts of the seedlings.
[0018] (2) Group setting: 6 groups were set up, wherein 5 groups were experimental groups, and the other group was a control group, with 100 plants in each group;
[0019] Experimental group: spraying diethyl sulfate (DES, mutagen) solution (pH value=7), each group concentration is respectively, A group: 0.05%; B group: 0.1%; C group: 0.15%; D group : 0.2%; Group E: 0.25%;
[0020] Control group: Spray 8-10ml of water;
[0021] It is advisable that the spraying amount of the experimental group and the control group is all moist on the whole plant leaf surface of each plant (the spraying amount of each seedling is...
Embodiment 2
[0033] Embodiment 2 verification test
[0034] The plant seeds (L) harvested by mutagenesis in the experimental group and the seeds harvested in the control group were sown together to raise seedlings. When the two types of plants were 45-50 days old, 20 vigorous and robust plants were selected and placed in the transplanted 38°C-40°C, 3-4 days in a light incubator with a relative humidity of 80%-90% (before being placed in the incubator, water each plant thoroughly, and do not water during high temperature stress). The plants in the control group had heat damage, and the leaves were extremely curled and shrunk. Some plants were partially wilted and yellow, and did not resume growth or even died after watering; normal growth.
[0035] Take the normal and disease-free leaves of the upper parts of the two types of plants (get the leaves of the same or similar parts), and measure the contents of three indicators such as malondialdehyde, peroxidase (POD) and free proline respecti...
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