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An active targeting nanosphere for photothermal treatment of tumors and its preparation method and application

A technology of active targeting and photothermal therapy, applied in the field of medicine, can solve the problems of poor treatment effect and large side effects of drugs, and achieve the effects of stable and reliable method, overcoming large side effects and stable performance.

Inactive Publication Date: 2017-12-22
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide an active targeting nanosphere for photothermal treatment of tumors and its preparation method and application, which can effectively solve the problem of large side effects and low therapeutic effect of existing tumor treatment drugs. bad question

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  • An active targeting nanosphere for photothermal treatment of tumors and its preparation method and application

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Embodiment 1

[0016] In concrete implementation, the present invention is realized by the following steps:

[0017] 1) Synthesis of copper sulfide nanoparticles: weigh 0.4mmoL CuCl 2 Dissolve in 8mL of povidone (PVP) solution with a concentration of 0.2g / mL, and form a light blue solution under magnetic stirring; another 1.2mmoL Na 2 Dissolve S in 8mL ultrapure water to form Na 2 S solution, the light blue solution and Na 2 The S solutions were combined, placed in a reaction kettle, reacted in a water bath at 180°C for 12h, and centrifuged at 1200r / min for 10min to obtain a black-green precipitate, which was washed 3 times with 40mL ethanol, dried under vacuum at 60°C to form copper sulfide nanoparticles ( PVP-Cu 2-x S);

[0018] 2) Preparation of passive targeting nanospheres: Weigh 6 mg copper sulfide nanoparticles (PVP-Cu 2-x S) Dissolve in 8 mL of acetic acid solution containing chitosan, the mass concentration of the acetic acid solution is 1%, the content of chitosan in the aceti...

Embodiment 2

[0021] The present invention can also be realized by the following steps in concrete implementation:

[0022] 1) Synthesis of copper sulfide nanoparticles: weigh 0.5mmoL CuCl 2 Dissolve in 10mL of povidone (PVP) solution with a concentration of 0.2g / mL, and form a light blue solution under magnetic stirring; another 1.5mmoL Na 2 Dissolve S in 10mL ultrapure water to form Na 2 S solution, the light blue solution and Na 2 The S solutions were combined, placed in a reaction kettle, reacted in a water bath at 180°C for 12h, and centrifuged at 1200r / min for 10min to obtain a black-green precipitate, which was washed 3 times with 50mL ethanol, and dried in vacuum at 60°C to form copper sulfide nanoparticles ( PVP-Cu 2-x S);

[0023] 2) Preparation of passive targeting nanospheres: Weigh 7.5 mg copper sulfide nanoparticles (PVP-Cu 2-x S) Dissolve in 10 mL of acetic acid solution containing chitosan, the mass concentration of the acetic acid solution is 1%, the content of chitosa...

Embodiment 3

[0026] The present invention can also be realized by the following steps in concrete implementation:

[0027] 1) Synthesis of copper sulfide nanoparticles: weigh 0.6mmoL CuCl 2 Dissolve in 12mL of povidone (PVP) solution with a concentration of 0.2g / mL, and form a light blue solution under magnetic stirring; another 1.8mmoL Na 2 Dissolve S in 12mL ultrapure water to form Na 2 S solution, the light blue solution and Na 2 The S solutions were combined, placed in a reaction kettle, reacted in a water bath at 180°C for 12h, and centrifuged at 1200r / min for 10min to obtain a black-green precipitate, which was washed 3 times with 60mL ethanol, dried under vacuum at 60°C to form copper sulfide nanoparticles ( PVP-Cu 2-x S);

[0028]2) Preparation of passive targeting nanospheres: Weigh 9 mg copper sulfide nanoparticles (PVP-Cu 2-x S) Dissolve in 12 mL of acetic acid solution containing chitosan, the mass concentration of the acetic acid solution is 1%, the content of chitosan in...

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Abstract

The invention relates to active targeting nanospheres for photothermal treatment of tumors and its preparation method and application, which effectively solve the problems of large side effects and poor therapeutic effects of existing tumor treatment drugs. The method is to dissolve CuCl2 in a povidone solution, Dissolve Na2S in ultrapure water, combine CuCl2 solution and Na2S solution, precipitate and dry to form copper sulfide nanoparticles, dissolve in acetic acid solution containing chitosan, and form PVP‑Cu2‑xS solution; take docetaxel solution In methanol, add to PVP‑Cu2‑xS solution, then add sodium tripolyphosphate, precipitate into passive targeting nanospheres; take folic acid and add passive targeting nanospheres, add 1‑ethyl‑(3‑dimethyl Aminopropyl) carbodiimide hydrochloride is centrifuged to remove the supernatant to obtain active targeting nanospheres. The method of the present invention is stable and reliable, and the resulting product has stable performance, convenient use, good effect, easy operation, and source of raw materials Rich, low cost, less side effects, can effectively inhibit the proliferation of tumor cells.

Description

technical field [0001] The invention relates to medicine, in particular to an active targeting nanosphere for photothermal treatment of tumors and its preparation method and application. Background technique [0002] The pathophysiological characteristics of tumor tissue are significantly different from those of normal tissue, mainly manifested in the strong vascular permeability of the tumor site. Macromolecular drugs and carriers can penetrate the vascular endothelial cells and enter the tumor tissue through the penetration and retention enhancement effect (that is, the EPR effect). It can accumulate in the tumor site for a long time and at a high concentration. Nanoparticles (NPs), including nanospheres and nanocapsules, are loaded or encapsulated drug molecules into nanomaterials, using the targeting, sustained release and EPR effect of the tumor site, through intravenous injection The nanoparticles are delivered to the tumor site to achieve the purpose of efficient tar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/14A61K31/337A61P35/00
Inventor 张振中祝侠丽张英杰黄河清侯琳张慧娟李鸿基
Owner ZHENGZHOU UNIV