Fermented milk drink rich in fructosan and production method thereof
A fermented milk beverage and fermented milk technology, which is applied in the fields of fermentation, dairy products, milk preparations, etc., can solve problems such as artificial addition of polysaccharides, achieve the effects of simplifying production steps, saving production costs, and reducing pollution risks
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Embodiment 1
[0031] Embodiment 1 is rich in the preparation of the fermented milk beverage of fructan
[0032] 1. Materials and methods
[0033] Preparation of skimmed milk sucrose medium: mix 10% skimmed milk powder with 15% sucrose by mass with distilled water, fully dissolve, sterilize at 125°C for 5min, and cool to room temperature to obtain the required concentration sterile skim milk sucrose medium.
[0034] Preparation of seeds (fermentation strains): the lyophilized powder of Leuconostoccitreum (Leuconostoccitreum) BD01707 whose preservation number is GCMCCNo. / v) On the M17 agar medium (MerckCo. Germany) of sucrose, 28 ℃ of aerobic cultures were taken out for 24h, and a single colony was picked with an inoculation loop and put into 20mL M17 liquid medium containing 2% (w / v) sucrose (purchased From Merck Co. (Germany), 28°C, 180rpm shaker culture for 24h, the culture was centrifuged at 9,000rpm for 10 minutes, the supernatant was discarded, the bacteria were washed twice with steri...
Embodiment 2
[0062] Embodiment 2 is rich in the preparation of the fermented milk beverage of fructan
[0063] 1. Materials and methods
[0064] Preparation of skim milk sucrose medium: mix skim milk powder with a mass percentage of 12%, sucrose with a mass percentage of 20% and distilled water, fully dissolve, sterilize at 95°C for 20min, and cool to room temperature to obtain the obtained Sterile skimmed milk sucrose medium of the desired concentration.
[0065] The preparation of seeds (fermented strains), the preparation of polysaccharides (fructan) in fermented milk, and the preparation of polysaccharides (fructan) in fermented milk drinks: the same as in Example 1.
[0066] 2. Preparation of fermented milk beverage rich in fructan
[0067] (1) The obtained Leuconostoc citrea BD01707 seeds are aseptically inoculated in a medium containing 20% sucrose and 12% skim milk with an inoculum volume percentage of 0.5%, cultivated at 35°C and 100rpm for 120 hours, and after the fermentatio...
Embodiment 3
[0070] Embodiment 3 is rich in the preparation of the fermented milk beverage of fructan
[0071] 1. Materials and methods
[0072] Preparation of skimmed milk sucrose medium: mix skimmed milk powder with a mass percentage of 6%, sucrose with a mass percentage of 5% and distilled water, fully dissolve, sterilize at 100°C for 15 minutes, and cool to room temperature to obtain the obtained Sterile skimmed milk sucrose medium of the desired concentration.
[0073] The preparation of seeds (fermented strains), the preparation of polysaccharides (fructan) in fermented milk, and the preparation of polysaccharides (fructan) in fermented milk drinks: the same as in Example 1.
[0074] 2. Preparation of fermented milk beverage rich in fructan
[0075] (1) Aseptically inoculate the obtained Leuconostoc citricum BD01707 seeds with an inoculum size of 4.0% (v / v) on a medium with a sucrose mass percentage content of 5% and a skim milk mass percentage content of 6%, at 20°C and 300rpm Af...
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