Preparing method of mixture of anglerfish stomach protein peptide and licorice powder
A technology of anglerfish and stomach protein, which is applied in the preparation of the mixture of anglerfish stomach protein peptide and licorice powder, and in the field of polypeptide preparation, can solve the problems of long digestion time, low product added value, and large gastrointestinal burden, etc. value, increased beneficial effect, good flavor effect
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[0029] In a typical embodiment of the present invention, a preparation method of a mixture of anglerfish pepsin peptide and licorice powder specifically includes:
[0030] Specific process flow: Anglerfish stomach-pretreatment-hydrolysis-enzyme inactivation-filtration-ingredients (adding licorice extract)-filtrate concentration-sterilization-spray drying-packaging.
[0031] (1) Pretreatment of fish stomach: the frozen fish stomach is thawed naturally, and then soaked in 1-10% NaOH, and mechanically stirred for 5-20 minutes at the same time. After the sewage is removed, the fish stomach is rinsed with water, and finally the fish stomach is twisted The meat machine grinds into granules with a particle size of 3-15mm.
[0032] (2) Hydrolysis: add water to the reactor, add the crushed particles, the material-to-liquid ratio is 1:1-1:5, the pH is controlled at pH 6.0-8.0, the temperature is controlled at 30-60°C, the first to be added during hydrolysis Papain, add enzyme amount 500-10000...
Embodiment 1
[0045] (1) Pretreatment of fish stomach: the frozen fish stomach is thawed naturally, then soaked in 1% NaOH, and mechanically stirred for 20 minutes at the same time. After the sewage is removed, the fish stomach is rinsed with water, and finally the fish stomach is minced with a meat grinder Into 3mm particles.
[0046] (2) Hydrolysis: add water to the reactor, add the crushed particles, the material-to-liquid ratio is 1:1, the pH is controlled at pH 6.0, and the temperature is controlled at 30°C. During hydrolysis, papain is first added, and the amount of enzyme added is 500U / g, add acid protease after reacting for 1h. The amount of enzyme added is 10000U / g. After reacting at 40℃ for 2h, the reaction is finished, and then the enzymolysis solution is heated to 90℃ for 10min to inactivate the enzyme.
[0047] (3) Filtration: the enzymatic hydrolysate is introduced into the plate and frame filter for filtration, the filter cake is discarded, and the filtrate is pumped into the buf...
Embodiment 2
[0058] (1) Pretreatment of fish stomach: the frozen fish stomach is thawed naturally, then soaked in 10% NaOH, and mechanically stirred for 5 minutes at the same time. After the sewage is removed, the fish stomach is rinsed with clean water, and finally the fish stomach is minced with a meat grinder The particle size is 15mm.
[0059] (2) Hydrolysis: add water to the reactor, add the crushed particles, the material-to-liquid ratio is 1:1, the pH is controlled at pH 6.0, and the temperature is controlled at 30°C. During the hydrolysis, papain is first added, and the amount of enzyme added is 10000U / g, add acid protease after reaction for 2h, the amount of enzyme added is 500U / g, after 0.5h at 60℃, the reaction is finished, then the enzymolysis solution is heated to 90℃ for 30min to inactivate the enzyme.
[0060] (3) Filtration: the enzymatic hydrolysate is introduced into the plate and frame filter for filtration, the filter cake is discarded, and the filtrate is pumped into the b...
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