Sphingobium sp. IBY and application thereof in adsorbing and degrading hydrophobic organics
A technology of adsorption and degradation, sphingolipid bacteria, applied in the field of microorganisms, can solve the problems of cell surface hydrophobicity difference, cell surface hydrophobicity is not high, does not belong to hydrophobic bacteria, etc., to achieve the effect of strong cell surface hydrophobicity and high survival rate
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Embodiment 1
[0018] Example 1: Isolation and identification of Sphingobium sp. IBY
[0019] Take 20g of riverbed sediment from an electronic waste centralized treatment area in Shantou, Guangdong, add 80mL of sterile water to stir and disperse, and then sieve to remove plant residues and large particles; the filtrate is then centrifuged at 2000×g at room temperature for 2 minutes to remove small particles ; The obtained supernatant was centrifuged at 6000×g at room temperature for 10 minutes to collect the precipitate; the precipitate was added to 80 mL of sterile water, washed and centrifuged three times. The finally obtained precipitate was used as a seed source and inoculated into inorganic salt medium (Na 2 HPO 4 5.7mM, KH 2 PO 4 3.3mM, NH 4 Cl 18.0mM, yeast extract 0.2g / L, β-cyclodextrin 10mM), 1μM of BDE209 and 2.8g / L of zero-valent iron powder were added to the medium at the same time. Add 20g / L agar to the solid medium. The culture was placed at 30°C and kept in the dark for ...
Embodiment 2
[0025] Example 2: Cell Surface Hydrophobicity of Sphingobium sp. IBY
[0026] The cell surface hydrophobicity of bacteria was determined by the microbially attached hydrocarbon (MATH) method. Single clones of strains (Sphingobium sp. IBY or Sphingobium xenophagum BN6) were picked from the plate and cultured overnight at 30°C with shaking in 5mL LB liquid medium. Transfer the overnight culture to fresh LB culture medium according to the inoculum amount of 1%, and cultivate it with shaking at 30°C until the middle and late stages of the logarithmic growth phase of the bacteria. Centrifuge at 9000×g for 10 min to collect the bacterial pellet, wash twice with phosphate buffered saline PBS (pH7.0), and finally resuspend the bacterial cell to OD with phosphate buffered saline PBS (pH7.0) 600 = 0.4. Add 3mL of bacterial suspension to the glass test tube, measure the OD at this time 600 value, denoted as OD 600 1. Add 0.15mL of n-hexadecane, vortex for 30s and let stand for 45min...
Embodiment 3
[0029] Embodiment 3: the survivability of Sphingobium sp. (Sphingobium sp.) IBY in organic solvent
[0030] Pick a single clone of the strain from the plate and place it in 5mL LB liquid medium, culture it overnight at 30°C with shaking. Transfer the overnight culture to fresh LB culture medium according to the inoculum amount of 1%, and cultivate it with shaking at 30°C until the middle and late stages of the logarithmic growth phase of the bacteria. Centrifuge at 9000×g for 10 min to collect the bacterial pellet, wash twice with phosphate buffered saline PBS (pH7.0), and finally resuspend the bacterial cell to OD with phosphate buffered saline PBS (pH7.0) 600 = 0.4. Add 3mL of bacterial suspension and 0.50mL of n-hexadecane to the glass test tube, vortex for 30s and let it stand still, take appropriate amount of bacterial liquid for electron microscope observation at regular intervals, and take appropriate amount of bacterial liquid to coat LB plates and culture at 30°C , ...
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