Check patentability & draft patents in minutes with Patsnap Eureka AI!

A kind of culture medium and method for direct somatic embryogenesis and plant regeneration of honeysuckle

A rooting medium and medium technology, applied in the field of plant biology, can solve problems not related to somatic embryogenesis, achieve the effects of enhancing plant stress resistance, high regeneration efficiency, and ensuring normal rooting

Active Publication Date: 2017-07-07
HUNAN ACAD OF FORESTRY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above patents are all limited to organogenesis, bud multiplication or rooting of honeysuckle. The composition of the culture medium is different due to organogenesis, bud multiplication or rooting. The basic medium is B 5 , Improved B 5 or 1 / 2MB with addition of 6-BA, NAA, KT, IAA, IBA, activated carbon, etc., but none of them involved somatic embryogenesis and honeysuckle plants regenerated by somatic embryogenesis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The direct somatic embryogenesis plant regeneration method of the honeysuckle is as follows:

[0078] The aseptic leaves of the honeysuckle variety "Jin Cuilei" were cut into 1 square centimeters each, and inoculated in direct somatic embryo induction medium. After 80 days, the total number of somatic embryos induced and the number of somatic embryos that had induced cotyledons were counted. Transfer to the rooting medium, count the rooting rate, rooting number and root length after 80 days; the culture condition of direct somatic embryo induction medium is: dark culture for 2 weeks, then carry out light culture, the light time is 12 hours every day, and the light intensity is 3850Lux, the temperature is 25°C; the rooting medium culture conditions are: light culture, the light time is 12 hours a day, the light intensity is 3850Lux, the temperature is 25°C; the regenerated plantlets are hardened for 1 week, and then transplanted into the substrate for cultivation , the s...

Embodiment 2

[0136] Step is the same as embodiment 1, and object is honeysuckle kind " Shundi No. 1 ";

[0137] The direct somatic embryo induction medium of 1000ml is made up of the raw material of following ratio:

[0138] Ammonium nitrate 1200 mg

[0139] Potassium nitrate 900 mg

[0140] Magnesium sulfate 180.7 mg

[0141] Calcium chloride 332.2 mg

[0142] Potassium dihydrogen phosphate 170 mg

[0143] Ferrous Sulfate Heptahydrate 27.8 mg

[0144] EDTA disodium salt 37.26 mg

[0145] Boric acid 6.2 mg

[0146] Zinc sulfate heptahydrate 8.6 mg

[0147] Manganese sulfate monohydrate 16.9 mg

[0148] Potassium iodide 0.83 mg

[0149] Sodium molybdate dihydrate 0.25 mg

[0150] Cobalt chloride hexahydrate 0.025 mg

[0151] Copper sulfate pentahydrate 0.025 mg

[0152] Potassium silicate 200 mg

[0153] Nickel sulfate hexahydrate 0.005 mg

[0154] Inositol 100 mg

[0155] Glycine 2.0 mg

[0156] Niacin 0.5 mg

[0157] Pyridoxine hydrochloride 0.5 mg

[0158] Thiamine hydr...

Embodiment 3

[0195] Step is the same as embodiment 1, and the object is the honeysuckle variety " Huayao late ripening ";

[0196] The direct somatic embryo induction medium of 1000ml is made up of the raw material of following ratio:

[0197] Ammonium nitrate 1200 mg

[0198] Potassium nitrate 900 mg

[0199] Magnesium sulfate 180.7 mg

[0200] Calcium chloride 332.2 mg

[0201] Potassium dihydrogen phosphate 170 mg

[0202] Ferrous Sulfate Heptahydrate 27.8 mg

[0203] EDTA disodium salt 37.26 mg

[0204] Boric acid 6.2 mg

[0205] Zinc sulfate heptahydrate 8.6 mg

[0206] Manganese sulfate monohydrate 16.9 mg

[0207] Potassium iodide 0.83 mg

[0208] Sodium molybdate dihydrate 0.25 mg

[0209] Cobalt chloride hexahydrate 0.025 mg

[0210] Copper sulfate pentahydrate 0.025 mg

[0211] Potassium silicate 200 mg

[0212] Nickel sulfate hexahydrate 0.005 mg

[0213] Inositol 100 mg

[0214] Glycine 2.0 mg

[0215] Niacin 0.5 mg

[0216] Pyridoxine hydrochloride 0.5 mg

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a culture medium and method for direct somatic embryogenesis and plant regeneration of honeysuckle. The aseptic leaves of honeysuckle are inoculated on the direct somatic embryo induction medium, cultured in the dark for two weeks, and then cultured under light. Embryos develop to the stage of cotyledon embryos. After a certain number of cotyledon embryos are induced, they are transferred to the rooting medium for further cultivation, and further develop into robust regenerated plantlets. The present invention is a honeysuckle somatic embryo propagation technology with simple operation, high efficiency and low cost, which has high frequency of occurrence, no deformed embryos, robust growth of tissue culture seedlings, and can be widely used in the cultivation of honeysuckle group seedlings, with a survival rate of over 99%. The production cost is reduced by more than 40%. Popularization and application of the invention will greatly improve the supply capacity of the fine seedlings of honeysuckle, and the economic benefit is remarkable.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and relates to a culture medium and a method for direct somatic embryogenesis and plant regeneration of honeysuckle. Background technique [0002] Honeysuckle contains more than 60 kinds of chemical components such as chlorogenic acid and luteolin. It has the effects of clearing away heat and detoxification, reducing inflammation and swelling, enhancing the body's immunity, and delaying aging. It also has anti-cancer and anti-AIDS effects. It is mainly used in clinical prevention and treatment Cardiovascular and cerebrovascular diseases, treatment of hepatitis, etc. It has wide adaptability, strong stress resistance, and no strict requirements on soil. It is an excellent landscaping plant, and it is also a good water and soil conservation and vegetation restoration tree species. In recent years, honeysuckle has the problems of backward varieties and degraded seedling quality. Although there ar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈建军李永欣王晓明曾慧杰蔡能乔中全
Owner HUNAN ACAD OF FORESTRY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com