Mixed biological organic selenium fertilizer, preparation method and applications thereof

An organic selenium fertilizer and organic selenium technology, which are applied in the fields of fertilizer technology and crop planting, can solve the problems of low yeast selenium enrichment and transformation efficiency, and achieve the effects of increasing dietary intake and stable selenium enrichment effect.

Active Publication Date: 2015-12-09
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0006] Aiming at the problems in the above-mentioned prior art that most of the organic selenium fertilizers are mainly obtained by mixing organic fertilizers and inorganic selenium, and the enrichment and conversion efficiency of pure yeast selenium in plants is low, the present invention provides a Compound biological organic selenium fertilizer, preparation method and use thereof

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  • Mixed biological organic selenium fertilizer, preparation method and applications thereof
  • Mixed biological organic selenium fertilizer, preparation method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Preparation of compound biological organic selenium fertilizer:

[0054] (1) Strain screening:

[0055] Using plasma mutagenesis technology, screen the sodium selenite and ethionine resistant mutants of Saccharomyces cerevisiae, and select yeast with larger colonies and lighter color for the next round of mutagenesis. After 3 rounds of mutagenesis screening A yeast mutant strain was obtained, the highest resistance to sodium selenite reached 5 mg / mL, and the highest resistance to ethionine reached 10 mg / mL.

[0056] (2) Preparation of selenium-enriched yeast:

[0057] Inoculate the high-selenium-enriched yeast mutant strain obtained through strain screening and inorganic selenium into the optimized YPD medium, and culture it at 30°C with a vibration frequency of 135r / min for 45h, and measure the OD of the fermentation broth at intervals of 3-4h during the period value, then add inorganic selenium according to the OD value, until the thallus grows to the logarithmic gr...

Embodiment 2

[0064] Except for 4 rounds of mutagenesis in step (1); in step (2), culture at 32°C and a vibration frequency of 120r / min for 40h, stop adding inorganic selenium until the selenium concentration in the finally obtained selenium-enriched yeast is 800ug / mL; step (3) enzymolysis under aseptic condition 20 ℃, ultrasonic 40min; Add ammonium nitrate (N45%) 40g / L, calcium phosphate (P 2 o 5 40%) 50g / L, chelating agent N-beta-hydroxyethylethylenediaminetriacetic acid 5g / L, potassium sulfate 10g / L, boric acid 10g / L and organic selenium, other processes are all identical with embodiment 1.

[0065] The selenium content in the compound bio-organic selenium solution prepared in this example is 810ug / mL, and the total selenium in the prepared compound bio-organic selenium fertilizer is 23.1g / kg, N is 3.2wt%, and P is 3.55wt%. , K is 3.1wt%, and B is 1.3wt%.

Embodiment 3

[0067] In addition to step (2) in 26 ℃, 150r / min vibration frequency for 48h, stop adding inorganic selenium until the selenium concentration in the finally obtained selenium-enriched yeast is 500ug / mL; in step (3) in sterile conditions 30 ℃ Under enzymolysis, ultrasonic 50min; Add calcium ammonium nitrate (N55%) 20g / L, calcium superphosphate (P 2 o 5 55%) 30g / L, chelating agent diethyltriaminepentaacetic acid 1g / L, plant ash 5g / L, borax 5g / L and organic selenium, other processes are all identical with embodiment 1.

[0068] The selenium content in the compound bio-organic selenium solution prepared in this example is 515ug / mL, the total selenium in the prepared compound bio-organic selenium fertilizer is 22.6g / kg, N is 3.54wt%, and P is 3.45wt%. , K is 3.45wt%, and B is 1.61wt%.

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Abstract

The invention provides a mixed biological organic selenium fertilizer, a preparation method, and applications thereof. The fertilizer comprises the following elements in percentage by weight: 2.0 to 3.0% of Se, not less than 2.0% of N, not less than 3.0% of P, not less than 2.0% of K, and not less than 0.5% of B. The preparation method comprises the following steps: screening bacterium strains, preparing selenium-enriched yeast, breaking the walls of the selenium-enriched yeast, and compounding the biological organic selenium fertilizer. The provided mixed biological organic selenium fertilizer can be applied to the production of selenium-enriched rice. The production and growth of paddy rice can be promoted, the output of rice can be increased by 5-10%; the selenium content of produced rice can reach 0.2 mg/kg or more, the national standards can be met, moreover, the selenium enriching effect is stable, the fertilizer can be applied to massive and standard production; and the selenium-enriched rice can increase the selenium intake amount for people.

Description

technical field [0001] The invention belongs to the field of fertilizer technology and crop planting technology, and in particular relates to a compound bio-organic selenium fertilizer, a preparation method and its use, in particular to a compound bio-organic selenium fertilizer applied to rice planting, a preparation method and its use . Background technique [0002] Selenium is a very important trace element in the ecological environment. It is one of the essential nutrients determined by the United Nations Health Organization. More than 40 kinds of diseases in the human body, including selenium, are directly related to the lack of selenium in the body. [0003] The Chinese Nutrition Society and the FAO / WHO / IAEA Joint Expert Committee have determined that the dietary selenium supply is 50 μg / day, and the appropriate range is 50-250 μg / day (equivalent to 0.1×10 whole blood selenium). -6 ~0.44×10 -6 ). But now the selenium intake of adults in my country is only 26-32 mic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00
Inventor 王凤忠范蓓黄亚涛卢嘉李敏敏
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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