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Method for rapidly measuring content of four spermidine ingredients in carthamus tinctorius simultaneously

A rapid determination of spermidine technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., to achieve the effect of reliable and easy operation of the method

Inactive Publication Date: 2015-12-16
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And there is no report about the content determination method of spermidines in the safflower, especially the method of rapid determination of four kinds of spermidine contents in the safflower simultaneously has no report

Method used

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  • Method for rapidly measuring content of four spermidine ingredients in carthamus tinctorius simultaneously
  • Method for rapidly measuring content of four spermidine ingredients in carthamus tinctorius simultaneously
  • Method for rapidly measuring content of four spermidine ingredients in carthamus tinctorius simultaneously

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Experimental program
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Effect test

Embodiment 1

[0019] Liquid chromatography analysis conditions: the analytical column is AgilentC 18 Chromatographic column (250mm×4.6mm, 5μm), the mobile phase is 47% methanol / water isocratic elution, the elution flow rate is 1.0ml / min, the column temperature is 30°C, the detection wavelength is 270nm, 280nm, 290nm, 300nm multi-wavelength For detection, the injection volume was 10 μl.

[0020] Preparation of reference solution:

[0021] Preparation of mixed reference substance stock solution: Accurately weigh 4.16 mg of the standard substance of spermidine compound 1, 5.12 mg of the standard substance of spermidine compound 2, 5.40 mg of the standard substance of spermidine compound 3, and 5.40 mg of the standard substance of spermidine compound 3. 4 Standard substance 10.04 mg, in a 100ml brown volumetric flask, was adjusted to the mark with methanol to obtain a mixed reference substance solution stock solution (each standard substance was prepared by our laboratory).

[0022] Preparati...

Embodiment 2

[0059] 1) Weigh 1.0000g of safflower powder (through a 60-mesh sieve), place it in a 100ml brown conical flask with a stopper, add 15ml of methanol, weigh it, and perform ultrasonic treatment (ultrasonic frequency 40KHz, power ratio 91%, ultrasonic temperature (25°C) for 10 minutes, let cool, weigh again, make up for the lost weight with methanol, shake well, and filter to obtain the safflower test solution, which is then tested after passing through a 0.45 μm microporous membrane;

[0060] 2) measure with Agilent liquid chromatograph, chromatographic condition: analytical column is AgilentC 18 Chromatographic column (250mm×4.6mm, 5μm), the mobile phase is 47% methanol / water isocratic elution, the elution flow rate is 1.0ml / min, the column temperature is 30°C, and the detection wavelength is 270nm, 280nm, 290nm, 300nm. The sample volume was 10 μl; the peak areas corresponding to the four spermidine components were obtained;

[0061] 3) Put the peak areas corresponding to the ...

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Abstract

The invention discloses a method for rapidly measuring content of four spermidine ingredients in carthamus tinctorius simultaneously and belongs to the technical field of measurement of content of effective components in a traditional Chinese medicine. Specifically, HPLC (high performance liquid chromatography) is adopted, and the liquid chromatogram analysis conditions are shown as follows: octadecylsilane chemically bonded silica is adopted as a filling agent, an organic solvent-water solution is adopted as a moving phase, isocratic elution is adopted, the detection wavelength is 210-360 nm, and the column temperature is 30 DEG C. The method is reliable and easy to operate, and a new method is provided for measurement of the content of spermidine. The method has an application prospect in the aspects of extraction, synthesis and analysis of spermidine and quality control during research, development and production of related drugs.

Description

technical field [0001] The invention relates to the technical field of content determination of effective components of traditional Chinese medicines, in particular to a method for simultaneously and rapidly determining the content of four spermidine components in safflower. Background technique [0002] Safflower is the dried tubular flower of Carthamustinctorius L. in the family Asteraceae. The flower tube is narrow and thin, about 1.5 cm long, orange-red. Its original name is red and blue flower, and its aliases are safflower cherry, grass safflower, thorn safflower, Du safflower, safflower and so on. The main production areas are Xinjiang, Henan, Zhejiang, and Sichuan provinces. It is pungent, slightly bitter, warm in nature, guixin, and liver meridian. It can promote blood circulation, relieve pain, reduce blood pressure, and lower blood lipids. It is used to treat amenorrhea and dysmenorrhea. , Angina pectoris, high blood pressure, bruises, coronary heart disease, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 李石飞袁茂叶张立伟
Owner SHANXI UNIV