Preparation method of water-soluble MnS nano-particles, and use of nano-particles as magnetic resonance imaging contrast agent
A nanoparticle and aqueous solution technology, applied in the direction of nanotechnology, nanotechnology, chemical instruments and methods, etc., can solve the problems of low relaxation efficiency and stability, and achieve good imaging effects, low toxicity, and high longitudinal relaxation efficiency Effect
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Embodiment 1
[0040] Step 1: Weigh 0.0252gMnCl 2 (0.2mmol) and 0.0452g thioacetamide (0.6mmol), put in a clean small beaker, add 3mL octanol, 3mL octylamine, 3mL acetone, 1mL oleic acid in sequence, stir for 10 minutes to make it evenly mixed, Then move it into the polytetrafluoroethylene liner of the 20mL reactor and fill it with N 2 5 minutes, expel the air in the inner lining. After tightening the lid of the kettle, put the reaction kettle into a 150-degree electric blast drying oven and keep it for 60 minutes. After the reaction, the reactor was taken out to cool down to room temperature naturally, centrifuged, and the supernatant was removed to obtain MnS precipitate.
[0041] Step 2: Disperse the MnS precipitate obtained in step 1 in 2mL cyclohexane, store it in an ampoule bottle with a polytetrafluoroethylene cover, shake it well and let it stand, the precipitate is well dispersed in cyclohexane, forming Brown yellow, homogeneous, transparent solution.
[0042] Step 3: Weigh 0.12...
experiment Embodiment 1
[0050] Experimental Example 1: Measurement of Relaxation Rate and Contrast Effect
[0051] 1. Dilute with water
[0052] The MnS aqueous solution prepared in Example 1 was diluted with secondary water into 5 solutions with different concentration gradients, and the solution concentrations were measured by ICP-MS as 0.063mM, 0.157mM, 0.314mM, 0.471mM, and 0.550mM, respectively. Weigh 5 samples respectively in small centrifuge tubes for later use. Turn on the RF switch of the MRIAnalyzing&Imagingsystem MicroMR20-025H-I instrument, set the queue name to Q-FID, put the standard oil sample into the quartz tube, put it into the 25mm probe coil, and adjust the parameters. The name of the queue is set to Q-IR, and then the standard oil sample is replaced with the sample with the lowest concentration, put into a quartz tube, and Tw (waiting time for repeated sampling) and NTI (number of inversion time) are obtained. Then replace the standard oil sample with the standard oil sample wi...
experiment Embodiment 2
[0060] Experimental Example 2: Cytotoxicity Experiment
[0061] HepG2 cells were cultured for 12 hours with different concentrations of MnS aqueous solution (0 μg / mL, 10 μg / mL, 30 μg / mL, 50 μg / mL, 70 μg / mL), and the cell survival rate was calculated.
[0062] (1), HepG2 cell culture
[0063] 1. Sow the cells in a 12-well plate, culture the cells with 10% FBS DMEM medium, and add MnS aqueous solution when the number of cells reaches about 50% of the 12-well plate.
[0064]2. The cells without MnS aqueous solution were used as the 0h cell number control, and the cells were treated with 0 μg / ml, 10 μg / ml, 30 μg / ml, 50 μg / ml, and 70 μg / ml of Mn for 12 hours.
[0065] (2), cell counting, data processing
[0066] After 1, 0h, and 12h of MnS aqueous treatment, the 10% FBS (fetal bovine serum) DMEM medium was aspirated, and then the cells were washed once with PBS (phosphate buffered saline solution), and the cells were fixed with 1ml of 4% paraformaldehyde.
[0067] 2. Wash away p...
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