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Preparation method and application of fermented Chinese herbal medicine water quality improver for aquaculture

A technology of fermented Chinese herbal medicine and water quality improver, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., which can solve the problems of complex components of Chinese herbal medicine, affecting application, and difficulty in extraction, and achieve inhibition of intestinal tract Effects of absorbing cholesterol, improving immunity, and enhancing immunity

Inactive Publication Date: 2017-06-06
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the complex components of Chinese herbal medicines, unclear pharmacological properties, difficult extraction, and poor water solubility have affected their application in aquaculture water environment and disease control.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: A fermented Chinese herbal medicine water quality improver for aquaculture and its preparation method adopts the following process steps:

[0032] (1) Cultivation of Bacillus subtilis: take a ring of Bacillus subtilis ( Basillus subtilis , CGMCC 1.1413, provided by the China General Microorganism Culture Collection Management Center) bacterial lawn, inoculated into 50mL sterilized broth medium, at 30°C, 120r min -1 Incubate on a shaker for 24 hours. Then put it into 5L of sterilized broth medium, at 30°C, 120r·min -1 Cultivate on a shaker for 24 hours, and prepare the strain seed solution for later use. OD of the bacterial solution at this time 600 The value is approximately equal to 1.0, and the number of viable bacteria is ≥10 8 CFU·mL -1 .

[0033] The broth liquid culture medium contains peptone 10g L -1 , beef extract 5g·L -1 , NaCl 5g·L -1 ,

[0034] Adjust the pH to 7.0, sterilize at 121°C for 20 minutes, and cool down for later use. ...

Embodiment 2

[0042] Embodiment 2: A fermented Chinese herbal medicine water quality improver and preparation method for aquaculture aquaculture adopt the following process steps:

[0043] (1) Cultivation of Bacillus subtilis: take a ring of Bacillus subtilis ( Basillus subtilis , CGMCC 1.1413, provided by the China General Microorganism Culture Collection Management Center) bacteria lawn, inoculated into 100mL sterilized broth medium, at 35°C, 100r min -1 Incubate on a shaker for 28 hours. Then put it into 8L of sterilized broth medium, at 35°C, 100r·min -1 Cultivate on a shaker for 28 hours, and prepare the strain seed solution for later use. OD of the bacterial solution at this time 600 The value is approximately equal to 1.0, and the number of viable bacteria is ≥10 8 CFU·mL -1 .

[0044] The broth liquid medium, containing peptone 10 g L -1 , beef extract 5 g·L -1 , NaCl 5 g·L -1 , adjust the pH to 7.2, sterilize at 121°C for 20 minutes, and cool down for later use.

[00...

Embodiment 3

[0052] Embodiment 3: A fermented Chinese herbal medicine water quality improver for aquaculture aquaculture and its preparation method adopts the following process steps:

[0053] (1) Cultivation of Bacillus subtilis: take a ring of Bacillus subtilis ( Basillus

[0054] subtilis , CGMCC 1.1413, provided by China General Microorganism Culture Collection Management Center) bacteria lawn, inoculated into 75mL sterilized broth medium, at 30 ℃, 110r min -1 Incubate on a shaker for 30 h. Then put it into 5L of sterilized broth medium, at 30°C, 110r min -1 Cultivate on a shaker for 30 hours, and prepare the strain seed solution for later use. OD of the bacterial solution at this time 600 The value is approximately equal to 1.0, and the number of viable bacteria is ≥10 8 CFU·mL -1 .

[0055] The broth liquid medium, containing peptone 10 g L -1 , beef extract 5 g·L -1 , NaCl 5 g·L -1 , adjust the pH to 7.0, sterilize at 121°C for 20 minutes, and cool down for later use. ...

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Abstract

The invention relates to a preparation method of a fermentation type Chinese herbal medicine water quality modifying agent for aquaculture and application thereof and belongs to the technical field of aquaculture. Two microbial strains, namely bacillus subtilis CGMCC 1.1413 and lactobacillus plantarum CGMCC 1.557 which have different characters and biological activities are mixed with houttuynia cordata, astragalus membranaceus and other Chinese herbal medicine with functions of resisting to bacteria and improving the immunity for fermentation, and the liquid fermentation type Chinese herbal medicine water quality modifying agent is obtained. By means of the preparation method, the water solubility and the bioavailability of the Chinese herbal medicine are effectively improved, microorganisms and Chinese herbal medicine are organically combined, use is convenient, the consumption is small, the minimum consumption of water surface per mu and eater depth per meter is only 0.5 kilogram, and influences of the environment conditions are avoided; the effect is stable after the agent is used, ammonia nitrogen and nitrite nitrogen in the aquatic water can be effectively removed or converted, pathogenic organisms such as vibriones can be restrained and antagonized, accumulation of harmful matter in the later period in the breeding process is reduced, the immunity of the bred organic bodies is improved, the purpose of biological water purification and disease resistance is achieved, the economic loss caused by disease is relieved, and the breeding yield and quality are guaranteed.

Description

technical field [0001] The invention relates to a preparation method and application of a fermented Chinese herbal medicine water quality improver for aquaculture, belonging to the technical field of aquaculture. Background technique [0002] Since the reform and opening up, my country's aquaculture industry has developed by leaps and bounds, and it has become the only fishery country in the world where the aquaculture output exceeds the fishing output. Most of the existing aquaculture industry in my country adheres to the traditional farming model characterized by "dense, mixed, and round" technology, that is, a farming model that obtains high yields with multi-species, high density, and large-scale fertilization and feeding. In order to increase the aquaculture output per unit of water body, water body nutrients are often excessively increased, excessive baiting, fertilization, and stocking density are increased, resulting in a sharp increase in nitrogen and phosphorus in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/25C02F103/20
Inventor 吴伟
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI