Biological degrading agent for degrading cyanide and preparing method and application of biological degrading agent
A technology of biodegradation agent and cyanide, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of destroying soil structure and biological communities, and achieve the effect of strong adsorption capacity
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Embodiment 1
[0040] Take 200g of peanut shells in a 500mL beaker, add 200ml of 10% ferric nitrate aqueous solution, soak for 12h, after the solution is completely blotted, place the obtained solid in a tube furnace, protect it with nitrogen, and calcinate at 450°C for 3h, after cooling Wash with 15% and 3% ammonium nitrate aqueous solutions in turn, then wash with deionized water for 3 times, dry at 105°C, pulverize, pass through a 120-mesh sieve, and sterilize with ozone for 10 minutes to obtain an activated carrier.
[0041] Take high-efficiency microbial flora B3501g and inoculate it in 100ml liquid medium. The formula of liquid medium is: KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O0.2g / L, CaCl 2 0.1g / L, NaCl0.2g / L, MnSO 4 ·H 2 O0.01g / L, FeCl 2 0.01g / L, NH 4 Cl1.0g / L; add potassium cyanide to make CN - The concentration is 2.0mg / L, and cultured in a shaker at 37°C for 3 days. After mycelium grows in the medium, 10ml of medium is supplemented every 3 days, and potassium ...
Embodiment 2
[0045] Take 100g of coconut peat into a 500mL beaker, add 150ml of 12% ferric phosphate aqueous solution, soak for 18h, after the solution is completely blotted, put the obtained solid in a tube furnace, protect it with nitrogen, calcinate at 400°C for 4h, cool After that, it was washed once with 20% and 4% ammonium phosphate aqueous solution successively, and then washed three times with deionized water, dried at 110°C, pulverized, passed through a 120-mesh sieve, and sterilized by ozone for 10 minutes to obtain an activated carrier.
[0046] Take high-efficiency microbial flora B3501g and inoculate it in 75ml liquid medium, the formula of liquid medium is: KH 2 PO 4 0.7g / L, K 2 HPO 4 0.7g / L, MgSO 4 ·7H 2 O0.1g / L, CaCl 2 0.2g / L, NaCl0.15g / L, MnSO 4 ·H 2 O0.03g / L, FeCl 2 0.02g / L, NH 4 Cl1.2g / L; add potassium cyanide to make CN - The concentration is 1.0mg / L, and cultured in a shaker at 35°C for 2 days. After mycelium grows in the medium, 15ml of medium is supplemente...
Embodiment 3
[0050] Take 500g of corn cobs in a 1000mL beaker, add 500ml of 8% magnesium nitrate aqueous solution, soak for 15h, after the solution is completely blotted, place the obtained solid in a tube furnace, protect it with nitrogen, and calcinate at 380°C for 4.5h. After cooling, it was washed once with 17% and 5% sodium nitrate aqueous solution, and then washed five times with deionized water, dried at 105°C, pulverized, passed through a 120-mesh sieve, and sterilized by ozone for 10 minutes to obtain an activated carrier.
[0051] Take 10g of high-efficiency microbial flora B350 and inoculate it in 1000ml liquid medium, the formula of liquid medium is: KH 2 PO 4 0.65g / L, K 2 HPO 4 0.65g / L, MgSO 4 ·7H 2 O0.2g / L, CaCl 2 0.15g / L, NaCl0.15g / L, MnSO 4 ·H 2 O0.01g / L, FeCl 2 0.02g / L, NH 4 Cl1.3g / L; add potassium cyanide to make CN - The concentration is 2.0mg / L, and cultured in a shaker at 37°C for 4 days. After mycelium grows in the medium, 300ml of medium is supplemented eve...
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