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Methods for Sequencing Immune Repertoires

A technology of immune group library and immunoglobulin, applied in the field of quantitative nucleic acid analysis

Active Publication Date: 2021-06-08
LINEAGE BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Although reference is made to binding specificity, and indeed a large number of serological assays are based on the physical interaction between antigen and receptor, the underlying cause of the diversity lies in the gene sequences expressed by lymphocytes, which reflect the recombination that has been imposed on the cells Innumerable processes of variation and selection

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  • Methods for Sequencing Immune Repertoires
  • Methods for Sequencing Immune Repertoires
  • Methods for Sequencing Immune Repertoires

Examples

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Embodiment Construction

[0022] The present invention provides a method of sequencing the improvement of the immunoholic library. The previous method of determining the immunohistocket library, for example, those methods based on multiple PCRs are described in WO 2011 / 140433 and WO 2012 / 083069. Multiple PCRs have many limitations that make it particularly useless to accurately determine the immunoholic library. (see figure 2 These limitations include capturing preferences and amplification preferences caused by PCR. Multiple PCR techniques of the sequencing immunohistochemistry are designed to initiate primers in all frame areas of known V gene fragments. When the mutation occurs at the initiation position, the capture preferences occur, and the mutated gene amplification is insufficient. PCR preferences are generated due to the relative amounts of PCR replicates having the same sequences. Therefore, PCR preferences can result in significant cloning or diversity lacking. In general, the observed group lib...

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Abstract

The present invention provides a non-invasive technique for the detection and quantification of the immune repertoire in a biological sample containing multiple distinct cell populations. The method is performed using sequencing technology to detect and enumerate immune repertoires in a xenogeneic biological sample.

Description

[0001] Related application [0002] The present invention requires the US Provisional Application No. 61 / 806143, filed on March 28, 2013, and the US Provisional Application No. 61 / 801785 submitted on March 15, 2013. All of its content is incorporated herein by reference This article. Technical field [0003] The present invention relates to a quantitative nucleic acid analysis field. More specifically, the present invention provides a method of determining an immuno group using a high-throughput sequence. Background technique [0004] The adaptive immune response is characterized by the ability to produce a wide variety of binding molecules, such as a T cell antigen receptor and an antibody. Various molecular mechanisms can be used to generate initial diversity, including gene recombination at multiple sites. The initial (NAIVE) B cells and initial T cells are equipped with initial groups of binding parts, which are circulated at a location that can be in contact with antigen. Whe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10C12Q1/6874C12Q1/6881
CPCC12N15/1096B01J19/0046B01J2219/00596B01J2219/00722C12Q2525/155C12Q2563/179C12Q2521/101C12Q2525/173C12Q2521/107C12Q1/6869C12Q2525/179C12Q1/6874C12Q1/6881C12Q2600/16
Inventor 爱德华·A·哈钦斯海-姆·克里斯蒂娜·范
Owner LINEAGE BIOSCI