Application of Proteinase K in the Field of Skin Care and Cosmetics
A protease, cosmetic technology, used in cosmetics, cosmetic preparations, preparations for skin care, etc.
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[0017] According to another embodiment of the present invention, as long as the mutant proteinase K retains the activity of the natural proteinase K, the mutant proteinase K can be used to prepare a cosmetic composition for removing aged keratinocytes. According to some embodiments of the present invention, the proteinase K mutant may be the proteinase K mutant disclosed in CN102839165A, which has the following mutations on the basis of natural proteinase K:
[0018] (1) In the native protease K amino acid sequence (SEQ ID NO.2), Y at position 151 is mutated to A, K at position 208 is mutated to H, S at position 273 is mutated to T, and G at position 293 is mutated A, K at position 332 is mutated to R, S at position 337 is mutated to N; optionally, any one or more of the following mutations are also performed:
[0019] (2) Mutation of S at position 123 to A;
[0020] (3) The L at position 180 is mutated into I.
[0021] In the present invention, "123 bits", "151 bits", "180 ...
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