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A kind of Fusarium aculinum and application thereof

A technology of Fusarium acuminate and its strains, applied in the field of Fusarium acuminate and its application, can solve the problems of accumulation of azo compounds, harm to humans and animals, etc.

Active Publication Date: 2018-08-24
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that after entering the human body, Sudan Red Ⅰ will be metabolized into corresponding anilines by the reductases of intestinal microorganisms and reductases in liver tissue and cytoplasm. These anilines are strongly mutagenic and carcinogenic. The industrial application of dye-like dyes will also lead to the accumulation of azo compounds in soil and urban water, and cause direct harm to humans and animals along with the concentration of the food chain
At present, there have been a lot of reports on the biodegradation of water-soluble azo dyes under anaerobic conditions, which have shown an important role in the decolorization and harmless treatment of dye wastewater, but the biodegradation of insoluble azo dyes There are few studies, so looking for microorganisms with the ability to degrade Sudan red dyes can be used to relieve the accumulation of toxic intermediate metabolites of Sudan red due to human and poultry metabolism, and to clarify the decolorization and degradation of non-water-soluble azo dyes. New metabolic pathways under aerobic conditions It is of great significance to reduce the pollution caused by the industrial application of such dyes to the environment.

Method used

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  • A kind of Fusarium aculinum and application thereof
  • A kind of Fusarium aculinum and application thereof
  • A kind of Fusarium aculinum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The isolation and identification of embodiment 1 Fusarium aculinum

[0027] 1. Separation

[0028] Cut the stems of healthy plant samples and wash them with clean water, soak in 75% ethanol for 1 min, then soak in 3.25% NaOCl for 5 min, put them in 75% ethanol again for 30 seconds, and finally rinse them with sterile water for 5 times. Samples were blotted dry with sterile absorbent paper for later use. Absorb 50 μL of the last washing water and spread them on PDA plates respectively, place them in an incubator at 28°C for 3 days, and observe whether there are colonies. Inoculate the surface-sterilized sample on PDA double-antibody medium, place it upside down in an incubator at 28°C and cultivate it for 15 days. After mycelium grows from the cut surface of each plant tissue, pick the edge part and move it to a new PDA solid medium, after purification endophytic fungi. Finally, it was inoculated on the slope of the PDA test tube and cultured at 28°C, and then it was ...

Embodiment 2

[0066] Example 2 The degradation of Fusarium acremonium B23 to Sudan Red I

[0067] Inoculate B23 into the dye medium with Sudan Red I concentrations of 50, 100, 150, 200, and 250ug / ml. The following is the degradation results, hyphae growth and degradation status of the bacteria under different Sudan Red concentrations. Two parameters are introduced: degradation efficiency μ and growth rate v; μ=diameter of hydrolysis circle / growth time, v=diameter of hyphae / growth time.

[0068] Such as figure 1 Shown is the degradation results of B23 cultured on different concentrations of Sudan Red I medium for 10 days. The white part in the medium is the degraded part, and the red part is the undegraded part. With the concentration of Sudan Red I in the medium Gradient increase, hydrolysis circle decreased significantly. figure 2 and image 3 Both the cell growth curve and the hydrolysis curve are close to a straight line. From figure 2 It can be seen from the figure that with the ...

Embodiment 3

[0069] Embodiment 3 Fusarium acremonium B23 is to the degradation condition of Sudan Red I

[0070] 1) Optimal carbon source optimization results

[0071] Since the growth rate of B-23 on the inorganic salt medium is too slow, the degradation time is too long, and the degradation efficiency is relatively low. Therefore, we hope to test whether the degradation rate of Sudan red by B-23 can be improved by adding different carbon sources. A single-factor experiment to determine the most suitable type of carbon source is carried out. The results are shown in Table 1 below. The degradability is represented by "+", the more the amount, the better the degradability, and no phenomenon is represented by "-".

[0072] Table 1: Carbon source single factor experimental record sheet

[0073]

[0074] After the five carbon sources of maltose, lactose, sucrose, glucose and starch, the order of B23 in the culture medium was: starch>maltose>glucose>sucrose>lactose. However, the degradatio...

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PUM

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Abstract

The invention provides fusarium acuminatum and application thereof. The bacterial strain is preserved already, the preservation institution is China Center for Type Culture Collection (CCTCC), the address is Wuhan University, the accession date is December 30, 2014, and the accession number is CCTCC NO: M2014678. The bacterial strain has dual functions of absorbing and degrading tonyred dyes. The study shows that when 5% lactose is added to the inorganic salt culture medium, the degrading effect is the most obvious; after the culture is carried out at 28 DEG C for 7 days, the degradation rate reaches 93.72%; as a newly developed biological agent, the fusarium acuminatum has quite good application prospects.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Fusarium aculinum and an application thereof. Background technique [0002] Plant endophytic fungi are an important class of microbial resources, which have important commercial and scientific value in the development of new drugs, protection of plant species, and interaction with hosts. At present, there have been many reports on the biodegradation of water-soluble azo dyes by bacteria, but there are few studies on the biodegradation of water-insoluble azo dyes. [0003] Sudan red is a kind of artificially synthesized chemical dyes, which are water-insoluble azo compounds, and are widely used in solvents, engine oils, waxes, rubber, shoe polish, and colorants in textiles and cosmetics. Sudan Red Ⅰ has attracted widespread social attention due to its potential carcinogenic hazards when added to salted duck eggs, chili oil and other foods. Studies have shown...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/77A62D101/28
Inventor 涂璇邓张双郭志勇向贤邹坤
Owner CHINA THREE GORGES UNIV
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