Mycobacterium phlei oral administration nanoparticles and preparation method therefor
A technology of mycobacterium phlei and nanoparticles, applied in the field of medicine, can solve the problem of destroying the efficacy of mycobacterium phlei, achieve the effect of being suitable for large-scale production, easy production control, and avoiding discomfort reactions
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[0041] The preparation method of oral Mycobacterium phlei nanoparticles of the present invention includes the following steps:
[0042] A. Dissolve the carrier material in an organic solvent to form an organic phase;
[0043] B. Add the Mycobacterium phlei solution dropwise to the organic phase to form colostrum;
[0044] C. Add the colostrum dropwise to the water phase to form a double emulsion;
[0045] D. Volatilize the organic solvent in the double emulsion, collect the solid matter by centrifugation, wash with distilled water, and dry to obtain oral Mycobacterium phlei nanoparticles.
[0046] Among them, the reasons for the selection of colostrum forming conditions, double emulsion forming conditions, water phase, and organic parameters are as follows:
[0047] 1.1 Selection of methods for forming colostrum
[0048] Taking the particle size and polydispersity coefficient of the colostrum as indicators, different methods are used to form the colostrum. When the particle size of the co...
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