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A non-aflatoxin-producing Aspergillus flavus strain and its application in biological control of peanut aflatoxin contamination

An aflatoxin and aflatoxin strain technology, which is applied in the field of environmental biology, can solve problems such as limited production and application, and achieve the effects of reducing aflatoxin pollution, improving colonization ability, and inhibiting infection

Active Publication Date: 2018-09-04
OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But generally speaking, the biological control of peanut aflatoxin pollution is still very limited due to various factors, and it is almost still a blank in our country.

Method used

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  • A non-aflatoxin-producing Aspergillus flavus strain and its application in biological control of peanut aflatoxin contamination
  • A non-aflatoxin-producing Aspergillus flavus strain and its application in biological control of peanut aflatoxin contamination
  • A non-aflatoxin-producing Aspergillus flavus strain and its application in biological control of peanut aflatoxin contamination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Isolation, screening and identification of bacterial strains

[0023] (1) Surface sterilize the peanut kernels with 70% ethanol for 30 seconds, then soak the kernels with 1wt% NaClO for 2 minutes, wash them with sterile water for 3 times, dry the peanut kernels, and evenly arrange the peanut kernels on a sterile plastic petri dish Medium, 10 grains / dish, keep the space between the kernels, not close to each other; wrap the petri dish with the kernels in parafilm, carefully place it in the finishing box with added water at the bottom, cover the box lid, and keep at 30°C Culture 7d;

[0024] (2) Utilize sterilized tweezers to dip the yellow-green conidia produced on a small amount of seeds, fully suspend and serially dilute in Tween-20 containing 0.1wt%, take 10 -4 Coat the PDA plate with diluted conidia suspension, culture at 30°C for 2 days, transfer a little of the growing single colony to the center of fresh CYA and AFPA solid medium, the medium is at n...

Embodiment 2

[0032] Example 2: Application of Aspergillus flavus NAFFHN396 in biological control of peanut aflatoxin pollution

[0033] After the non-toxigenic Aspergillus flavus NAFFHN396 and the toxigenic Aspergillus flavus AF2202 were cultivated on PDA solid medium at 30°C for 7 days, the conidia were suspended in 0.1wt% Tween-20 water, and the number of conidia was counted by a hemocytometer , adjust the concentration of non-toxin-producing bacteria and toxin-producing bacteria to be the same 1*10 4 , at a ratio of 1:1, mixed in equal amounts and inoculated in the center of the CYA solid medium, or inoculated on the surface of the surface-sterilized peanut kernels, fully shaking the kernels to make the conidia evenly adhere to the surface of the kernels, and at the same time CYA solid medium or peanut kernels inoculated only with AF2202 were set up as a control, and cultured at 30°C for 7 days. The method described in Example 1 step (4) is used to extract the aflatoxin on the CYA soli...

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Abstract

The invention belongs to the field of the environmental biotechnology, and specifically relates to an aspergillus flavus strain incapable of producing aflatoxin and an application of the aspergillus flavus strain in biological prevention and control of aflatoxin pollution of peanuts. The aspergillus flavus strain incapable of producing aflatoxin is named as aspergillus flavus NAFFHN396, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 28, 2015, and the preservation serial number is CGMCC No: 10927. The aspergillus flavus strain incapable of producing aflatoxin with deletion of an aflatoxin synthetic cluster gene is obtained, and has an obvious inhibiting effect for the yield of the toxin of the strain with strong capacity of producing aflatoxin, thus a biocontrol bacterium source is provided for the biological prevention and control of the toxic aspergillus flavus generated in the field in the future, the aspergillus flavus strain is separated from China, has good colonization capacity in peanuts and potential field competitive advantage, and has great significances in inhibiting infection of the peanuts by the toxin production aspergillus flavus, and reducing the pollution of the aflatoxin in the peanuts.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to an aspergillus flavus strain not producing aflatoxin and its application in the biological control of peanut aflatoxin pollution. Background technique [0002] Aflatoxin is a very important mycotoxin that endangers food safety in my country. In nature, some crops, including corn and peanuts, are susceptible to infection by Aspergillus flavus, resulting in aflatoxin contamination of the grain; other plants, such as nuts, are also susceptible to aflatoxin contamination. Aflatoxins are highly toxic and are the most toxic carcinogenic mycotoxins known so far. They are extremely toxic to the liver and central nervous system of humans and animals. Long-term intake of small doses can lead to embryonic deformities, mutations, Gene and chromosomal mutations can induce primary liver cancer, gastric cancer and other cancers, and one-time large intake can cause acu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/67
Inventor 晏立英廖伯寿雷永万丽云康彦平
Owner OIL CROPS RES INST CHINESE ACAD OF AGRI SCI