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Applications of Cochinchinoid A in preparing medicines for treating breast cancer

A breast cancer and drug technology, applied in the application field of CochinchinoidA in the preparation of breast cancer drugs, can solve the problems such as no reports of breast cancer activity

Inactive Publication Date: 2016-01-13
吴正锋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no report on the activity of this compound on breast cancer

Method used

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  • Applications of Cochinchinoid A in preparing medicines for treating breast cancer
  • Applications of Cochinchinoid A in preparing medicines for treating breast cancer
  • Applications of Cochinchinoid A in preparing medicines for treating breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Isolation, Preparation and Structure Confirmation of Cochinchinoid A

[0013] The preparation method of Cochinchinoid A was the same as that reported in the literature (11β-HSD1 Inhibitors from Walsuracochinchinensis, J. Nat. Prod., 2013, 76, 1319-1327).

[0014] Confirmation of structure: colorless crystal, melting point 205-207°C, molecular formula C 36 h 50 o 9 , with an unsaturation of 12. The absorption band of the infrared spectrum indicates that the compound contains a hydroxyl group (3433cm -1 ) and carbonyl (1743 and 1712cm -1 ) group. H NMR spectrum data δ H (ppm, DMSO-d 6 , 600MHz): H-1 (3.52, ddd, J=8.7, 3.2, 2.4), H-2 (2.31, m), H-2 (2.01, ddd, J=16.3, 2.8, 2.4), H-3 (5.07, dd, J=2.8, 2.8), H-5 (2.35, d, J=12.5), H-6 (4.20, dd, J=12.5, 3.2), H-7 (5.68, d, J= 3.2), H-9 (2.86, dd, J=11.8, 7.3), H-11 (1.79, 1.81, m), H-12 (1.61, ddd, J=13.2, 9.8, 2.9), H-12 ( 2.44, m), H-15 (5.93, s), H-18 (1.01, s), H-19 (0.99, s), H-21 (7.57, dd, J=1.7, ...

Embodiment 2

[0015] Embodiment 2: Pharmacological action test of CochinchinoidA

[0016] 1. Materials and Instruments

[0017] DMEM / F12 high glucose culture medium was purchased from Beijing Hyclone Company. The improved RPMI-l640 medium was purchased from Beijing Hyclone Company. Standard fetal bovine serum was purchased from Tianjin TBD Company. CCK-8 cell viability assay reagents were purchased from Tongjin Chemical Research Institute, Japan. AnnexinV-FITC / PI apoptosis detection kit was purchased from Shanghai Yanbin Chemical Technology Co., Ltd. JC-1 mitochondrial membrane potential probe was purchased from Jiangsu Biyuntian Biotechnology Research Institute. Dimethylsulfite (DMSO) and phosphate buffered saline (PBS) were purchased from Sigma. CochinchinoidA is self-made, and the HPLC normalized purity is greater than 98%.

[0018] -70°C low temperature refrigerator (Forma Scientific Inc., USA), electronic balance (Shimadzu AEL-200 type, Japan), ultra-clean bench (SW-CJ-IC type, S...

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Abstract

The invention discloses applications of Cochinchinoid A in preparing medicines for treating breast cancer, belonging to the field of medicines. The study on the functions of Cochinchinoid A in resisting breast cancer shows that Cochinchinoid A enables the proliferative activity of three breast cancer cells to be reduced, obviously inhibits the proliferation of the cells, shows the relatively strong in-vitro cytotoxicity, has the in-vitro breast cancer resisting effect, and can be further studied and developed to be prepared into the medicines for treating breast cancer.

Description

technical field [0001] The present invention relates to a new application of compound Cochinchinoid A, in particular to the application of Cochinchinoid A in the preparation of drugs for treating breast cancer. Background technique [0002] Mei-LingHan et al. isolated and purified the compound Cochinchinoid A for the first time, and published the results in the famous Journal of Natural Products (11β-HSD1 Inhibitors from Walsuracochinchinensis, J.Nat.Prod., 2013, 76, 1319-1327). In this paper, the inhibitory activity of the compound on 11β-HSD1 was studied, cochinchinoidK has inhibitory activity on mouse 11β-HSD1, IC 50 The value is 0.82 μM. [0003] There is no report about the activity of this compound on breast cancer so far. Contents of the invention [0004] The purpose of the present invention is to provide a medical application of CochinchinoidA. [0005] The above-mentioned purpose is achieved through the following technical solutions: [0006] The application ...

Claims

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Application Information

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IPC IPC(8): A61K31/58A61P35/00
Inventor 吴正锋
Owner 吴正锋