Method for purification preparation of N-sulfocarbamoyl toxins

A sulfonylcarbamoyl and toxin technology, which is applied in the field of microorganism detection, can solve the problem of no systematic method for the cultivation of toxin-producing alga AT, and achieves the effect of simple method and high toxin purity.

Inactive Publication Date: 2016-01-13
SHANGHAI ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there is still no systematic and optimal me

Method used

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  • Method for purification preparation of N-sulfocarbamoyl toxins
  • Method for purification preparation of N-sulfocarbamoyl toxins
  • Method for purification preparation of N-sulfocarbamoyl toxins

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Embodiment 1

[0025] 1. Materials and methods

[0026] 1.1 Main instruments and reagents

[0027] LC-MS / MS: Electrospray ionization source (ESI) (ThermoFisher Scientific, USA);

[0028] HPLC post-column fluorescence derivatization system: Watersalliance2690 / 2475 (Waters, USA);

[0029] Gel filtration system: J2preplincGPC / SPE / evap combined instrument (J2scientific, USA);

[0030] Incubator: PQX-350H artificial climate box, Shanghai Shenxian Constant Temperature Equipment Factory;

[0031] Digital PH meter: PHS-3C, Shanghai Leici Instrument Factory;

[0032] Freeze dryer: HetoPowerDryLL3000, Shanghai Huifen Electronic Technology Co., Ltd.;

[0033] Cell pulverizer: GA98-IIIDB, Wuxi Shangjia Biotechnology Co., Ltd.;

[0034] Handheld refractometer: OTC, Shanghai Tianlei Instrument Co., Ltd.

[0035] 1.2 Standards and reagents

[0036] Toxin standards (STX, NEO, GTX1 / 4, GTX2 / 3, C1 / C2) were purchased from the National Research Council of Canada Marine Life Science Institute; formic acid,...

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Abstract

The invention provides a method for purification preparation of N-sulfocarbamoyl toxins. The method comprises the steps of culture of toxin production algae, extraction of toxins, collection and purification of the toxins and the like. The process is simple and effective, meanwhile, the purity of the prepared N-sulfocarbamoyl gonyautoxins-2 (C1) and N-sulfocarbamoyl gonyautoxins-3 (C2) can reach higher than 91%, and the N-sulfocarbamoyl toxins can be used for quality safety detection and scientific research of saxitoxin in aquatic products.

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a method for purifying and preparing N-sulfonylcarbamoyl toxin. Background technique [0002] Paralytic shellfish toxins (PSTs) are a class of highly water-soluble small molecule secondary metabolites produced by planktonic algae or microorganisms in water bodies. Its basic structure is two guanidine-based four-hydrated purine stacked six-membered rings. According to the different substituent groups, it is mainly divided into four categories: ① Carbamate toxins (Carbamatetoxins), including saxitoxin (STX), neoSTX (NEO) and genital toxin 1 -4 (Gonyautoxins, GTX1-4); ②N-sulfonylcarbamoyltoxins (N-sulfocarbamoyltoxins), including GTX5(B1), GTX6(B2) and C1-C4; ③Decarbamoyltoxins (Decarbamoyltoxins) , including dcSTX, dcneoSTX, dcGTX1-4; ④ deoxydecarbamyl toxoids (Deoxydecarb-amoyltoxins), including doSTX, doneoSTX and doGTX1 and so on. [0003] PSTs are a class of highly eff...

Claims

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Application Information

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IPC IPC(8): C07D487/16C12N1/12C12R1/89
CPCC07D487/16C12N1/12
Inventor 杨宪立周磊聂冬霞赵志勇武爱波
Owner SHANGHAI ACAD OF AGRI SCI
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