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The use of abcc4 gene polymorphism site rs3742106 and its detection primers and kit

A technology of gene polymorphism and kit, applied in the field of medicine

Active Publication Date: 2020-04-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polymorphism site rs3742106 of the ABCC4 gene is a SNP site located on the 3'-UTR of the ABCC4 gene. At present, no studies have shown that the polymorphism site rs3742106 of the ABCC4 gene is related to the efficacy of chemotherapy drugs

Method used

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  • The use of abcc4 gene polymorphism site rs3742106 and its detection primers and kit
  • The use of abcc4 gene polymorphism site rs3742106 and its detection primers and kit
  • The use of abcc4 gene polymorphism site rs3742106 and its detection primers and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] A kit and method for predicting the chemotherapy efficacy of colorectal cancer patients based on single base extension-MALDI / TOF / MS method to determine the genotype of ABCC4 gene polymorphism site rs3742106.

[0083] 1. The composition of the kit:

[0084] 1. Primers for detecting the rs3742106 genotype of the ABCC4 gene polymorphism site;

[0085] Primers for amplifying polymorphic loci:

[0086] SEQ ID No. 1: 5'-CAAGATGGTGCAACAACTGG-3'

[0087] SEQ ID No.2: 5'-GTTAGAGCTGGAGATCCTTG-3'

[0088] Primers for single base extension of polymorphic loci:

[0089] SEQ ID No.4: 5'-AGTCCGTTCCGAAGGCATTT-3'

[0090] 2. PCR amplification enzyme and corresponding buffer;

[0091] 3. dNTPs;

[0092] 4. PCR product purification enzyme;

[0093] 5. Single base extension reaction enzyme and corresponding buffer;

[0094] 6. Enzyme purification of single base extension products.

[0095] 2. Genotype detection and curative effect prediction:

[0096] 1. Collection of specimens: ...

Embodiment 2

[0127] A kit and method for determining the genotype of the ABCC4 gene polymorphism site rs3742106 based on DNA sequencing.

[0128] 1. The composition of the kit:

[0129] 1. Primers for amplifying the polymorphic site rs3742106 of the ABCC4 gene:

[0130] SEQ ID No. 1: 5'-CAAGATGGTGCAACAACTGG-3'

[0131] SEQ ID No.2: 5'-GTTAGAGCTGGAGATCCTTG-3'

[0132] 2. PCR amplification enzyme and corresponding buffer;

[0133] 3. dNTPs;

[0134] 4. PCR product purification enzyme.

[0135] 2. Genotype detection

[0136] 1. Collect specimens

[0137] 1ml of whole blood was drawn from the patient's peripheral vein and placed in a 0.5M EDTA anticoagulant tube.

[0138] 2. Genomic DNA extraction

[0139] Use the Genomic DNA Extraction Kit to extract DNA according to the instructions.

[0140] 3. Genotype detection

[0141] (1) Take 1 μl of DNA sample, and use 1% agarose gel electrophoresis to check the quality of the sample.

[0142] (2) Take 1 μl of qualified DNA sample, and use ...

Embodiment 3

[0158] A kit and method for determining the genotype of the ABCC4 gene polymorphism site rs3742106 based on restriction fragment length polymorphism analysis.

[0159] 1. The composition of the kit:

[0160] 1. Primers for amplifying the polymorphic site rs3742106 of the ABCC4 gene:

[0161] SEQ ID No.2: 5'-GTTAGAGCTGGAGATCCTTG-3'

[0162] SEQ ID No.3: 5'-AGTCCGTTCCGAAGGCAATT-3'

[0163] 2. PCR amplification enzyme and corresponding buffer;

[0164] 3. dNTPs;

[0165] 4. Restriction endonuclease and its reaction buffer.

[0166] 2. Genotype detection

[0167] 1. Collect specimens

[0168] 1ml of whole blood was drawn from the patient's peripheral vein and placed in a 0.5M EDTA anticoagulant tube.

[0169] 2. Genomic DNA extraction

[0170] Use the Genomic DNA Extraction Kit to extract DNA according to the instructions.

[0171] 3. Genotype detection

[0172] (1) Take 1 μl of DNA sample, and use 1% agarose gel electrophoresis to check the quality of the sample.

[01...

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Abstract

The invention relates to the field of medicines, and particularly relates to the application of ABCC4 gene polymorphic site rs3742106 as well as a detection primer and a kit thereof. Tests prove that colorectum cancer chemotherapeutic effect can be predicted according to polymorphic site rs3742106 genotype: when the genotype is AA, the chemotherapeutic effect of a patient with colorectum cancer is predicted to be good, and when the genotype is CC or CA, the chemotherapeutic effect is bad. Therefore, the invention provides the application of the ABCC4 gene polymorphic site rs3742106 in preparing products for predicting the colorectum cancer chemotherapeutic effect, and provides a primer group and the kit for detecting the ABCC4 gene polymorphic site rs3742106, and a detection method. By adopting the kit and the method, provided by the invention, the polymorphic site rs3742106 can be accurately measured, the colorectum cancer chemotherapeutic effect can be predicted, and a predicting result is in line with the expectation.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of ABCC4 gene polymorphism site rs3742106 and its detection primers and kit. Background technique [0002] Colorectal cancer (CRC) is one of the most common malignancies. Worldwide, the incidence of CRC ranks third and fourth among malignant tumors in males and females, and the mortality rate ranks fourth and fifth, respectively. In recent years, the incidence of CRC in my country has shown an obvious upward trend, especially in developed coastal areas and large and medium-sized cities. According to statistics, the annual average number of new CRC cases in my country reaches 130,000, accounting for about 12% to 15% of systemic tumors, and continues to rise with an average annual growth rate of 4%. Due to its rapid development, high degree of malignancy, and difficult treatment, CRC has been paid more and more attention by the medical field, especially the domestic medica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6886
Inventor 汪维鹏王蕾陈琦邹玲婷
Owner SUZHOU UNIV