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Detection method for fingerprint chromatogram of flavonoid and organic acid components in ginkgo biloba extract and application of detection method

A technology of ginkgo ketone ester and fingerprint spectrum is applied in the field of analysis and detection of traditional Chinese medicine components, and can solve the problems of affecting the intrinsic quality of medicines, increasing the content of total flavonol glycosides, and low flavonoid aglycones.

Active Publication Date: 2016-01-20
SPH XING LING SCI & TECH PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the fingerprint of Ginkgo biloba extract has not been compiled into the "Chinese Pharmacopoeia", the content of flavonoid aglycone in Ginkgo biloba extract itself is low. If flavonoid aglycone such as quercetin is artificially added, the total The content of flavonoid glycosides was significantly increased, which apparently met the requirements, but actually affected the internal quality of the drug

Method used

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  • Detection method for fingerprint chromatogram of flavonoid and organic acid components in ginkgo biloba extract and application of detection method
  • Detection method for fingerprint chromatogram of flavonoid and organic acid components in ginkgo biloba extract and application of detection method
  • Detection method for fingerprint chromatogram of flavonoid and organic acid components in ginkgo biloba extract and application of detection method

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Experimental program
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Effect test

Embodiment 1

[0100] 1. Experimental part

[0101] 1.1 Sample pretreatment

[0102] Preparation of the test solution: accurately weigh 100 mg of ginkgo ketone ester, place in a 15ml centrifuge tube, add 10-90% methanol aqueous solution, preferably 60% methanol aqueous solution, so that the solid-liquid ratio is 1:80-120 (g / ml), preferably adding 10ml of 60% methanol precisely (so that the ratio of solid to liquid is 1:100 (g / ml)), after airtight, at a rotation speed of 2500-3000r / min, preferably 2800r / min, vortex extraction for 3-10min is preferred for 5 minutes, filter with a filter membrane with a pore size of 0.45 μm, and take the subsequent filtrate to obtain the test solution.

[0103] Preparation of reference substance solution: accurately weigh an appropriate amount of rutin reference substance, accurately add 10-90% methanol aqueous solution, preferably 60% methanol aqueous solution, dissolve and constant volume, and prepare a reference substance solution with a known concentratio...

Embodiment 2

[0139] 1. Experimental part

[0140] 1.1 Sample pretreatment

[0141] The preparation of need testing solution: accurately weigh ginkgo ketone ester 100mg, place in 15ml centrifuge tube, add 60% methyl alcohol 10ml precisely (making solid-liquid ratio be 1: 100 (g / ml)), airtight after rotating speed is 2800r / min, vortex extraction for 5min, filter with a filter membrane with a pore size of 0.45μm, and take the subsequent filtrate to obtain the test solution.

[0142] Preparation of reference product solution: accurately weigh an appropriate amount of rutin reference product, accurately add 60% methanol aqueous solution to dissolve and constant volume, and prepare a reference product solution of known concentration. The concentration of rutin in the reference product solution is preferably 250 μg / ml.

[0143] 1.2 Chromatographic conditions

[0144] The chromatographic conditions of high performance liquid chromatography are: chromatographic column: Agilent Poroshell120SBC18 ...

Embodiment 3

[0171] 1. Experimental part

[0172] 1.1 Sample pretreatment

[0173] The preparation of need testing solution: accurately weigh ginkgo ketone ester 100mg, place in 15ml centrifuge tube, add 60% methyl alcohol 10ml precisely (making solid-liquid ratio be 1: 100 (g / ml)), airtight after rotating speed is 2800r / min, vortex extraction for 5min, filter with a filter membrane with a pore size of 0.45μm, and take the subsequent filtrate to obtain the test solution.

[0174] Preparation of the reference substance solution: Accurately weigh the reference substances of rutin, quercetin, kaempferol, and isorhamnetin respectively, accurately add 60% methanol aqueous solution to dissolve and constant volume, and obtain the first mixed mother solution; then precisely weigh the isorhamnetin Li Su reference substance, precisely add 60% methanol aqueous solution to dissolve and constant volume to obtain the second mixed mother liquor; then mix the first mixed mother liquor and the second mix...

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Abstract

The invention provides a detection method for a fingerprint chromatogram of flavonoid and organic acid components in a ginkgo biloba extract. The detection method comprises the following steps: (1) preparing a test solution; (2) preparing a reference solution; (3) respectively determining the test solution and the reference solution by adopting high-performance liquid chromatography, and comparing the acquired fingerprint chromatogram with a standard fingerprint chromatogram to obtain the fingerprint chromatogram of the flavonoid and organic acid components in the test solution. The invention further provides the application of the detection method in the quality detection and the component test determination of the flavonoid and organic acid components in the ginkgo biloba extract. According to the detection method for the fingerprint chromatogram of flavonoid and organic acid components in the ginkgo biloba extract and the application of the detection method provided by the invention, the fingerprint chromatogram of flavonoid and organic acid medicinal components in the ginkgo biloba extract is established; the quality control level of the flavonoid and organic acid medicinal components in the ginkgo biloba extract is improved; effective quantitative analysis can be performed.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection of traditional Chinese medicine components, and relates to a method for detecting fingerprints of flavonoids and organic acids in ginkgo ketone esters and its application, in particular to a fingerprint of flavonoids and organic acids in ginkgo ketone esters. Spectrum detection method and its application in content determination. Background technique [0002] Ginkgo biloba is the dried leaf of Ginkgo biloba plant. It is sweet, bitter, astringent, flat, heart-guiding and lung meridian. It is recorded in "Chinese Medicine Zhi" that ginkgo leaf can "contract lung qi, relieve cough and asthma, and stop turbidity". Ginkgo Biloba Extract (GEB50) is a new generation of ginkgo biloba extract product independently developed by my country. It has obtained the original national second-class new drug, and passed the US clinical pre-trial and entered the IND stage. It is mainly used for the trea...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 吴佩颖闫玉娇高崎顾慧芬
Owner SPH XING LING SCI & TECH PHARM CO LTD
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