A kind of dual stationary phase thin-layer chromatographic plate and its preparation method

Abstract

The invention provides a double-stationary-phase thin-layer chromatoplate and a preparation method therefor. The thin-layer chromatoplate comprises a base plate and a stationary phase thin layer. The chromatoplate also comprises a border line, a hydrophilic stationary phase thin layer and a hydrophobic stationary phase thin layer. The hydrophilic stationary phase thin layer and the hydrophobic stationary phase thin layer are porous, and attached at two sides of the border line on the base plate respectively, the thicknesses of the two thin layers are the same, and the two thin layers are in bonding connection at the border line. The invention also provides a preparation method for the double-stationary-phase thin-layer chromatoplate. The preparation method comprises the following steps: a hydrophilic polymer fluid and a hydrophobic polymer fluid are prepared; a hydrophilic stationary phase thin layer is prepared; part of the hydrophilic stationary phase thin layer is scraped, and a hydrophobic stationary phase thin layer is prepared at the scraped position; activation processing is carried out. The hydrophilic stationary phase thin layer and the hydrophobic stationary phase thin layer are prepared individually, mutual interference of control of pore diameters of the two thin layers is avoided, ideal pore diameters can be obtained, and therefore the separation effects of the chromatoplate are raised.

Description

technical field [0001] The invention relates to a dual-stationary-phase thin-layer chromatography plate and a preparation method thereof, belonging to the technical field of chromatography. Background technique [0002] Thin-layer chromatography uses a thin-layered support with a porous structure coated on the substrate as the stationary phase, and uses a suitable solvent as the mobile phase, and utilizes the different adsorption capabilities of each component for the same adsorbent to make the mobile phase (solvent) In the process of flowing through the stationary phase (adsorbent), adsorption, desorption, re-adsorption, and re-desorption are continuously generated, so as to achieve the purpose of mutual separation of various components. [0003] When the stationary phase is a strongly hydrophilic polar adsorbent, the TLC plate has the function of hydrophilic chromatography; when the stationary phase is a hydrophobic non-polar adsorbent, the TLC plate has the function of hy...

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Problems solved by technology

[0005] Photografting technology belongs to the method of post-synthesis modification. It can only connect other branches inside the pores of the original hydrophilic thin layer, which will make the new hydrophobic thin layer smaller than the original thin layer. Therefore, the entire thin layer The pore size distribution of the chromatographic plate is not uniform and uncontrollable

Since the pore size plays a decisive role in the chromatographic separation effect of the TLC plate, and the pore size in the stationary phase thin layer prepared by photografting technology is uneven and uncontrollable, it is difficult to achieve a pore size distribution with a good separation effect, which affects the dual Chromatographic Separation Effect of Stationary Phase TLC Plate

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