Monochromatic field microscope illuminating system

Abstract

The invention relates to a monochromatic field microscope illuminating system, and the system comprises a condenser outer cylinder, an annular lens, a condenser inner cylinder, a monochrome cylinder and a monochrome cylinder support. The annular lens and the condenser inner cylinder are arranged in the upper part of the condenser outer cylinder, a light transmission hole is arranged on the center of the annular lens and on the central optical axis, and an internal screw thread is on the lower end of the condenser outer cylinder. The condenser inner cylinder is arranged in the condenser outer cylinder through the screw thread, and the monochrome cylinder is fixed to the center of the condenser inner cylinder through the monochrome cylinder support. A central through hole of the monochrome cylinder is coaxially communicated with the light transmission hole of the annular lens, an upper screw thread and a lower screw thread are in the monochrome cylinder for assembling a neutral density attenuation slice and an optical filter respectively. According to the invention, the defects in the prior art including low light source utilization rate, low visibility of the imaging of the weak scattering plasma nano-particles and limited application are overcome, the scattering imaging visibility is increased, the relatively good imaging contrast ratio is retained, and the system is suitable for the imaging of different colors of the plasma nano-particles.

Description

technical field [0001] The invention relates to a microscope, in particular to an illumination system for the microscope, which is mainly used for plasma nanoparticle light scattering imaging. Background technique [0002] Ordinary optical microscopy provides high-brightness illumination to the imaging area to form a true projection of the internal structure of the specimen details, while dark-field microscopy uses light to illuminate the object from the side, diffraction or reflection forms the outline of the object or reveals the movement of the object. From the perspective of geometric optics, the imaging principle of the dark field microscope is explained as follows: the dark field condenser covers the incident light in the imaging area, converts the parallel light source into a ring beam with a large incident angle, and forms an inversion away from the imaging area after passing through the ring lens The light cone, the annular light beam shoots to the periphery of the ...

AI Technical Summary

Problems solved by technology

At the same time, this technology also has the following disadvantages: 1) only a small amount of light sources are involved in lighting, and the utilization rate of light sources is low; 2) for some plasmonic nanoparticles with weak scattering intensity, the imaging visibility is low under the existing technology

These defects will limit the imaging effect and application of dark field microscopy

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