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Culture medium for tissue culture seedlings of savia miltiorrhiza and rapid propagation method for savia miltiorrhiza tissue culture

A tissue culture rapid propagation and culture medium technology, applied in the field of plant tissue culture, can solve the problems of long growth cycle, high production cost, quality degradation, etc., and achieve the effect of short growth cycle, large production volume and less variation

Active Publication Date: 2016-02-10
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few wild resources of Salvia miltiorrhiza, and the traditional cultivation mode faces problems such as long growth cycle, quality degradation, and relatively high production cost.

Method used

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  • Culture medium for tissue culture seedlings of savia miltiorrhiza and rapid propagation method for savia miltiorrhiza tissue culture
  • Culture medium for tissue culture seedlings of savia miltiorrhiza and rapid propagation method for savia miltiorrhiza tissue culture
  • Culture medium for tissue culture seedlings of savia miltiorrhiza and rapid propagation method for savia miltiorrhiza tissue culture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The method for tissue culture and rapid propagation of Salvia miltiorrhiza, comprises the steps:

[0028] Step 1, the preparation of several MS medium mother solutions.

[0029] MS100 times the preparation of a large amount of element mother liquor, respectively weighed 190g of potassium nitrate, 165g of ammonium nitrate, 17g of potassium dihydrogen phosphate, 37g of magnesium sulfate, and 44g of calcium chloride, respectively added to five 1000mL beakers, added an appropriate amount of distilled water, stirred to dissolve, and fixed The capacity is 1000mL, and the five kinds of macroelement mother liquors are stored in the refrigerator for later use.

[0030] Preparation of MS1000 times trace element mother liquor, weigh 0.83g of potassium iodide, 6.2g of boric acid, 16.9g of manganese sulfate, 8.6g of zinc sulfate, 0.25g of sodium molybdate, 0.025g of copper sulfate, and 0.025g of cobalt chloride into a 1000mL beaker , add an appropriate amount of distilled water, st...

Embodiment 2

[0040] This patent investigates the influence of different plant growth regulators and concentrations on the induction of salvia miltiorrhiza tissue culture sprouts, the influence of culture medium composition and culture temperature on rooting (except for the investigation factors, other factors and conditions are equal to Example 1). Determined the optimal culture program of Danshen tissue culture seedlings. The results of the investigation are listed below.

[0041] Table 1 Effects of different plant growth regulators and concentrations on the induction of salvia miltiorrhiza tissue culture seedlings

[0042]

[0043]

[0044] It can be seen from Table 1 that when 6-BA1mg and NAA0.5mg are added per liter of medium, the induction effect on clustered buds is the best.

[0045] Table 2 Effects of different plant growth regulators and concentrations on rooting of Danshen tissue culture seedlings

[0046]

[0047] It can be seen from Table 2 that when 1 mg of IBA and ...

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Abstract

The invention discloses a culture medium for tissue culture seedlings of savia miltiorrhiza and a rapid propagation method for savia miltiorrhiza tissue culture. Savia miltiorrhiza stems with axillary bud seve as the materials, savia miltiorrhiza stem tissue culture and propagation are conducted, and the large number of high quality savia miltiorrhiza seedlings can be obtained through explant disinfection treatment, induction and differentiation culture, rooting culture and acclimatization and transplant. The culture medium and the rapid propagation method have the advantages that the growing cycle is short, the propagation rate is high, variation is little, the culture condition can be controlled artificially, operation is simple, output is large, a theoretical basis and a practice basis are provided for achieving the goal of producing the savia miltiorrhiza seedlings on a large scale and in an industrialized mode within a short period, and the foundation is laid for genetic transformation, breed improvement and the like of savia miltiorrhiza.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a tissue culture technology of salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza, also known as red ginseng, Zhuwu, Yuchancao, galloping horse grass, etc., is the dry root and rhizome of Salviamiltiorrhiza Bunge, a plant of the Labiatae family, with a bitter, slightly cold taste. Guixin, liver meridian, has the effects of promoting blood circulation and dredging collaterals, dispelling stasis and relieving pain, cooling blood and eliminating carbuncle, clearing heart and eliminating troubles, etc., and has significant effects on cardiovascular and blood systems. Clinically, it is mostly used to treat chest obstruction and heartache, epigastric and hypochondriac pain, accumulation of lumps in the abdomen, heat arthralgia pain, restlessness and insomnia, irregular menstruation, dysmenorrhea, sore carbuncle, swelling and pain. With the in-depth res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 蒲高斌张永清梁从莲李佳刘谦
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE